A central role for CK1 in catalyzing phosphorylation of the p53 transactivation domain at serine 20 after HHV-6B viral infection

Nicola J. MacLaine, Bodil Oster, Bettina Bundgaard, Jennifer A. Fraser, Carolyn Buckner, Pedro A. Lazo, David W. Meek, Per Höllsberg, Ted R. Hupp

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    Abstract

    The tumor suppressor protein p53 is activated by distinct cellular stresses including radiation, hypoxia, type I interferon, and DNA/RNA virus infection. The transactivation domain of p53 contains a phosphorylation site at Ser(20) whose modification stabilizes the binding of the transcriptional co-activator p300 and whose mutation in murine transgenics induces B-cell lymphoma. Although the checkpoint kinase CHK2 is implicated in promoting Ser(20) site phosphorylation after irradiation, the enzyme that triggers this phosphorylation after DNA viral infection is undefined. Using human herpesvirus 6B (HHV-6B) as a virus that induces Ser(20) site phosphorylation of p53 in T-cells, we sought to identify the kinase responsible for this virus-induced p53 modification. The p53 Ser(20) kinase was fractionated and purified using cation, anion, and dye-ligand exchange chromatography. Mass spectrometry identified casein kinase 1 (CK1) and vaccinia-related kinase 1 (VRK1) as enzymes that coeluted with virus-induced Ser(20) site kinase activity. Immunodepletion of CK1 but not VRK1 removed the kinase activity from the peak fraction, and bacterially expressed CK1 exhibited Ser(20) site kinase activity equivalent to that of the virus-induced native CK1. CK1 modified p53 in a docking-dependent manner, which is similar to other known Ser(20) site p53 kinases. Low levels of the CK1 inhibitor D4476 selectively inhibited HHV-6B-induced Ser(20) site phosphorylation of p53. However, x-ray-induced Ser(20) site phosphorylation of p53 was not blocked by D4476. These data highlight a central role for CK1 as the Ser(20) site kinase for p53 in DNA virus-infected cells but also suggest that distinct stresses may selectively trigger different protein kinases to modify the transactivation domain of p53 at Ser(20).

    Original languageEnglish
    Pages (from-to)28563-28573
    Number of pages11
    JournalJournal of Biological Chemistry
    Volume283
    Issue number42
    DOIs
    Publication statusPublished - 17 Oct 2008

    Keywords

    • DNA-DEPENDENT ACETYLATION
    • C VIRUS NS5A
    • COACTIVATOR P300
    • POINT MUTATION
    • KINASE
    • HYPERPHOSPHORYLATION
    • BINDING
    • DAMAGE
    • CELLS
    • SITE

    Cite this

    MacLaine, N. J., Oster, B., Bundgaard, B., Fraser, J. A., Buckner, C., Lazo, P. A., Meek, D. W., Höllsberg, P., & Hupp, T. R. (2008). A central role for CK1 in catalyzing phosphorylation of the p53 transactivation domain at serine 20 after HHV-6B viral infection. Journal of Biological Chemistry, 283(42), 28563-28573. https://doi.org/10.1074/jbc.M804433200