A chloroplast retrograde signal regulates nuclear alternative splicing

Ezequiel Petrillo; Micaela A. Godoy Herz; Armin Fuchs; Dominik Reifer; John Fuller; Marcelo J. Yanovsky; Craig Simpson; John W S Brown; Andrea Barta; Maria Kalyna; Alberto R. Kornblihtt

doi:10.1126/science.1250322

A chloroplast retrograde signal regulates nuclear alternative splicing

Ezequiel Petrillo, Micaela A. Godoy Herz, Armin Fuchs, Dominik Reifer, John Fuller, Marcelo J. Yanovsky, Craig Simpson, John W S Brown, Andrea Barta, Maria Kalyna, Alberto R. Kornblihtt (Lead / Corresponding author)

Plant Sciences

Research output: Contribution to journal › Article › peer-review

163 Citations (Scopus)

Abstract

Light is a source of energy and also a regulator of plant physiological adaptations. We show here that light/dark conditions affect alternative splicing of a subset of Arabidopsis genes preferentially encoding proteins involved in RNA processing. The effect requires functional chloroplasts and is also observed in roots when the communication with the photosynthetic tissues is not interrupted, suggesting that a signaling molecule travels through the plant. Using photosynthetic electron transfer inhibitors with different mechanisms of action, we deduce that the reduced pool of plastoquinones initiates a chloroplast retrograde signaling that regulates nuclear alternative splicing and is necessary for proper plant responses to varying light conditions.

Original language	English
Pages (from-to)	427-30
Number of pages	4
Journal	Science
Volume	344
Issue number	6182
DOIs	https://doi.org/10.1126/science.1250322
Publication status	Published - 25 Apr 2014

Keywords

Alternative Splicing
Arabidopsis
Arabidopsis Proteins
Cell Nucleus
Chloroplasts
Circadian Clocks
Dibromothymoquinone
Diuron
Electron Transport
Gene Expression Regulation, Plant
Light
Models, Biological
Oxidation-Reduction
Photosynthesis
Plant Leaves
Plant Roots
Plants, Genetically Modified
Plastoquinone
RNA Stability
RNA, Messenger
RNA, Plant
Seedling
Signal Transduction

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10.1126/science.1250322

Brown, John
- DArcy Thompson Unit - Honorary Professor (PhD Supervisor) of Molecular Plant Sciences
Person: Honorary

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title = "A chloroplast retrograde signal regulates nuclear alternative splicing",

abstract = "Light is a source of energy and also a regulator of plant physiological adaptations. We show here that light/dark conditions affect alternative splicing of a subset of Arabidopsis genes preferentially encoding proteins involved in RNA processing. The effect requires functional chloroplasts and is also observed in roots when the communication with the photosynthetic tissues is not interrupted, suggesting that a signaling molecule travels through the plant. Using photosynthetic electron transfer inhibitors with different mechanisms of action, we deduce that the reduced pool of plastoquinones initiates a chloroplast retrograde signaling that regulates nuclear alternative splicing and is necessary for proper plant responses to varying light conditions.",

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author = "Ezequiel Petrillo and {Godoy Herz}, {Micaela A.} and Armin Fuchs and Dominik Reifer and John Fuller and Yanovsky, {Marcelo J.} and Craig Simpson and Brown, {John W S} and Andrea Barta and Maria Kalyna and Kornblihtt, {Alberto R.}",

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doi = "10.1126/science.1250322",

language = "English",

volume = "344",

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T1 - A chloroplast retrograde signal regulates nuclear alternative splicing

AU - Petrillo, Ezequiel

AU - Godoy Herz, Micaela A.

AU - Fuchs, Armin

AU - Reifer, Dominik

AU - Fuller, John

AU - Yanovsky, Marcelo J.

AU - Simpson, Craig

AU - Brown, John W S

AU - Barta, Andrea

AU - Kalyna, Maria

AU - Kornblihtt, Alberto R.

PY - 2014/4/25

Y1 - 2014/4/25

N2 - Light is a source of energy and also a regulator of plant physiological adaptations. We show here that light/dark conditions affect alternative splicing of a subset of Arabidopsis genes preferentially encoding proteins involved in RNA processing. The effect requires functional chloroplasts and is also observed in roots when the communication with the photosynthetic tissues is not interrupted, suggesting that a signaling molecule travels through the plant. Using photosynthetic electron transfer inhibitors with different mechanisms of action, we deduce that the reduced pool of plastoquinones initiates a chloroplast retrograde signaling that regulates nuclear alternative splicing and is necessary for proper plant responses to varying light conditions.

AB - Light is a source of energy and also a regulator of plant physiological adaptations. We show here that light/dark conditions affect alternative splicing of a subset of Arabidopsis genes preferentially encoding proteins involved in RNA processing. The effect requires functional chloroplasts and is also observed in roots when the communication with the photosynthetic tissues is not interrupted, suggesting that a signaling molecule travels through the plant. Using photosynthetic electron transfer inhibitors with different mechanisms of action, we deduce that the reduced pool of plastoquinones initiates a chloroplast retrograde signaling that regulates nuclear alternative splicing and is necessary for proper plant responses to varying light conditions.

KW - Alternative Splicing

KW - Arabidopsis

KW - Arabidopsis Proteins

KW - Cell Nucleus

KW - Chloroplasts

KW - Circadian Clocks

KW - Dibromothymoquinone

KW - Diuron

KW - Electron Transport

KW - Gene Expression Regulation, Plant

KW - Light

KW - Models, Biological

KW - Oxidation-Reduction

KW - Photosynthesis

KW - Plant Leaves

KW - Plant Roots

KW - Plants, Genetically Modified

KW - Plastoquinone

KW - RNA Stability

KW - RNA, Messenger

KW - RNA, Plant

KW - Seedling

KW - Signal Transduction

U2 - 10.1126/science.1250322

DO - 10.1126/science.1250322

M3 - Article

C2 - 24763593

SN - 0036-8075

VL - 344

SP - 427

EP - 430

JO - Science

JF - Science

IS - 6182

ER -

A chloroplast retrograde signal regulates nuclear alternative splicing

Abstract

Keywords

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Brown, John

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