TY - JOUR
T1 - A compact Airy beam light sheet microscope with a tilted cylindrical lens
AU - Yang, Zhengyi
AU - Prokopas, Martynas
AU - Nylk, Jonathan
AU - Coll-Lladó, Clara
AU - Gunn-Moore, Frank J.
AU - Ferrier, David E. K.
AU - Vettenburg, Tom
AU - Dholakia, Kishan
N1 - We thank the UK Engineering and Physical Sciences Research Council under grant EP/J01771X/1, the ’BRAINS’ 600th anniversary appeal and Dr. E. Killick for funding.
PY - 2014/9/5
Y1 - 2014/9/5
N2 - Light-sheet imaging is rapidly gaining importance for imaging intact biological specimens. Many of the latest innovations rely on the propagation-invariant Bessel or Airy beams to form an extended light sheet to provide high resolution across a large field of view. Shaping light to realize propagation-invariant beams often relies on complex programming of spatial light modulators or specialized, custom made, optical elements. Here we present a straightforward and low-cost modification to the traditional light-sheet setup, based on the open-access light-sheet microscope OpenSPIM, to achieve Airy light-sheet illumination. This brings wide field single-photon light-sheet imaging to a broader range of endusers. Fluorescent microspheres embedded in agarose and a zebrafish larva were imaged to demonstrate how such a microscope can have a minimal footprint and cost without compromising on imaging quality.
AB - Light-sheet imaging is rapidly gaining importance for imaging intact biological specimens. Many of the latest innovations rely on the propagation-invariant Bessel or Airy beams to form an extended light sheet to provide high resolution across a large field of view. Shaping light to realize propagation-invariant beams often relies on complex programming of spatial light modulators or specialized, custom made, optical elements. Here we present a straightforward and low-cost modification to the traditional light-sheet setup, based on the open-access light-sheet microscope OpenSPIM, to achieve Airy light-sheet illumination. This brings wide field single-photon light-sheet imaging to a broader range of endusers. Fluorescent microspheres embedded in agarose and a zebrafish larva were imaged to demonstrate how such a microscope can have a minimal footprint and cost without compromising on imaging quality.
UR - http://www.scopus.com/inward/record.url?scp=84942374722&partnerID=8YFLogxK
U2 - 10.1364/BOE.5.003434
DO - 10.1364/BOE.5.003434
M3 - Article
AN - SCOPUS:84942374722
SN - 2156-7085
VL - 5
SP - 3434
EP - 3442
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 10
ER -
