A conserved oomycete CRN effector targets tomato TCP14-2 to enhance virulence

Remco Stam, Graham B. Motion, Victor Martinez Heredia, Petra Boevink, Edgar Huitema (Lead / Corresponding author)

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Abstract

Phytophthora spp. secrete vast arrays of effector molecules during infection to aid in host colonization. The crinkling and necrosis (CRN) protein family forms an extensive repertoire of candidate effectors that accumulate in the host nucleus to perturb processes required for immunity. Here, we show that CRN12_997 from Phytophthora capsici binds a TCP transcription factor, SlTCP14-2, to inhibit its immunity-associated activity against Phytophthora spp. Coimmunoprecipitation and bimolecular fluorescence complementation studies confirm a specific CRN12_997-SlTCP14-2 interaction in vivo. Coexpression of CRN12_997 specifically counteracts the TCP14- enhanced immunity phenotype, suggesting that CRN mediated perturbation of SlTCP14-2 function. We show that SlTCP14-2 associates with nuclear chromatin and that CRN12_997 diminishes SlTCP14-2 DNA binding. Collectively, our data support a model in which SlTCP14-2 associates with chromatin to enhance immunity. The interaction between CRN12_997 and SlTCP14-2 reduces DNA binding of the immune regulator. We propose that the modulation of SlTCP14-2 chromatin affinity, caused by CRN12-997, enhances susceptibility to P. capsici.
Original languageEnglish
Pages (from-to)309-318
Number of pages10
JournalMolecular Plant - Microbe Interactions
Volume34
Issue number3
Early online date1 Dec 2020
DOIs
Publication statusPublished - 3 Mar 2021

Keywords

  • Oomycete effectors
  • Oomycete-plant interactions
  • Phytophthora diseases

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