A current assessment of photosystem II structure

William V. Nicholson, Robert C. Ford, Andreas Holzenburg

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

This review covers the recent progress in the elucidation of the structure of photosystem II (PSII). Because much of the structural information for this membrane protein complex has been revealed by electron microscopy (EM), the review will also consider the specific technical and interpretation problems that arise with EM where they are of particular relevance to the structural data. Most recent reviews of photosystem II structure have concentrated on molecular studies of the PSII genes and on the likely roles of the subunits that they encode or they were mainly concerned with the biophysical data and fast absorption spectroscopy largely relating to electron transfer in various purified PSII preparations. In this review, we will focus on the approaches to the three-dimensional architecture of the complex and the lipid bilayer in which it is located (the thylakoid membrane) with special emphasis placed upon electron microscopical studies of PSII-containing thylakoid membranes. There are a few reports of 3D crystals of PSII and of associated X-ray diffraction measurements and although little structural information has so far been obtained from such studies (because of the lack of 3D crystals of sufficient quality), the prospects for such studies are also assessed.
Original languageEnglish
Pages (from-to)159-187
Number of pages29
JournalBioscience Reports
Volume16
Issue number2
DOIs
Publication statusPublished - Apr 1996

Fingerprint

Photosystem II Protein Complex
Thylakoids
Electron microscopy
Electron Microscopy
Electrons
Membranes
Crystals
Lipid bilayers
Lipid Bilayers
Absorption spectroscopy
X-Ray Diffraction
Spectrum Analysis
Membrane Proteins
Genes
X ray diffraction

Cite this

Nicholson, William V. ; Ford, Robert C. ; Holzenburg, Andreas. / A current assessment of photosystem II structure. In: Bioscience Reports. 1996 ; Vol. 16, No. 2. pp. 159-187.
@article{4b22283d9a8f42ae9bf6edd94d84571c,
title = "A current assessment of photosystem II structure",
abstract = "This review covers the recent progress in the elucidation of the structure of photosystem II (PSII). Because much of the structural information for this membrane protein complex has been revealed by electron microscopy (EM), the review will also consider the specific technical and interpretation problems that arise with EM where they are of particular relevance to the structural data. Most recent reviews of photosystem II structure have concentrated on molecular studies of the PSII genes and on the likely roles of the subunits that they encode or they were mainly concerned with the biophysical data and fast absorption spectroscopy largely relating to electron transfer in various purified PSII preparations. In this review, we will focus on the approaches to the three-dimensional architecture of the complex and the lipid bilayer in which it is located (the thylakoid membrane) with special emphasis placed upon electron microscopical studies of PSII-containing thylakoid membranes. There are a few reports of 3D crystals of PSII and of associated X-ray diffraction measurements and although little structural information has so far been obtained from such studies (because of the lack of 3D crystals of sufficient quality), the prospects for such studies are also assessed.",
author = "Nicholson, {William V.} and Ford, {Robert C.} and Andreas Holzenburg",
year = "1996",
month = "4",
doi = "10.1007/bf01206204",
language = "English",
volume = "16",
pages = "159--187",
journal = "Bioscience Reports",
issn = "0144-8463",
publisher = "Portland Press",
number = "2",

}

A current assessment of photosystem II structure. / Nicholson, William V.; Ford, Robert C.; Holzenburg, Andreas.

In: Bioscience Reports, Vol. 16, No. 2, 04.1996, p. 159-187.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A current assessment of photosystem II structure

AU - Nicholson, William V.

AU - Ford, Robert C.

AU - Holzenburg, Andreas

PY - 1996/4

Y1 - 1996/4

N2 - This review covers the recent progress in the elucidation of the structure of photosystem II (PSII). Because much of the structural information for this membrane protein complex has been revealed by electron microscopy (EM), the review will also consider the specific technical and interpretation problems that arise with EM where they are of particular relevance to the structural data. Most recent reviews of photosystem II structure have concentrated on molecular studies of the PSII genes and on the likely roles of the subunits that they encode or they were mainly concerned with the biophysical data and fast absorption spectroscopy largely relating to electron transfer in various purified PSII preparations. In this review, we will focus on the approaches to the three-dimensional architecture of the complex and the lipid bilayer in which it is located (the thylakoid membrane) with special emphasis placed upon electron microscopical studies of PSII-containing thylakoid membranes. There are a few reports of 3D crystals of PSII and of associated X-ray diffraction measurements and although little structural information has so far been obtained from such studies (because of the lack of 3D crystals of sufficient quality), the prospects for such studies are also assessed.

AB - This review covers the recent progress in the elucidation of the structure of photosystem II (PSII). Because much of the structural information for this membrane protein complex has been revealed by electron microscopy (EM), the review will also consider the specific technical and interpretation problems that arise with EM where they are of particular relevance to the structural data. Most recent reviews of photosystem II structure have concentrated on molecular studies of the PSII genes and on the likely roles of the subunits that they encode or they were mainly concerned with the biophysical data and fast absorption spectroscopy largely relating to electron transfer in various purified PSII preparations. In this review, we will focus on the approaches to the three-dimensional architecture of the complex and the lipid bilayer in which it is located (the thylakoid membrane) with special emphasis placed upon electron microscopical studies of PSII-containing thylakoid membranes. There are a few reports of 3D crystals of PSII and of associated X-ray diffraction measurements and although little structural information has so far been obtained from such studies (because of the lack of 3D crystals of sufficient quality), the prospects for such studies are also assessed.

U2 - 10.1007/bf01206204

DO - 10.1007/bf01206204

M3 - Article

VL - 16

SP - 159

EP - 187

JO - Bioscience Reports

JF - Bioscience Reports

SN - 0144-8463

IS - 2

ER -