A developmentally regulated position effect at a telomeric locus in Trypanosoma brucei

David Horn, George A.M. Cross

Research output: Contribution to journalArticlepeer-review

121 Citations (Scopus)

Abstract

Trypanosoma brucei undergoes antigenic variation in the mammalian host. This can be achieved by activation and inactivation of telomeric variant-specific surface glycoprotein genes (vsg). In procyclic (insect midgut stage) cells, Vsg is not expressed. The mechanisms that regulate transcription of vsg expression sites (ESs) are unknown. Here we demonstrate that transcription from three different promoters was repressed when they were inserted at a transcriptionally silent telomere-proximal locus in bloodstream-form cells. This position effect was stable and heritable. Only transcription from an ES promoter was repressed in procyclic cells. The observed position effect and the promoter-specific developmental regulation suggest that these phenomena reflect the mechanisms that regulate vsg expression.

Original languageEnglish
Pages (from-to)555-561
Number of pages7
JournalCell
Volume83
Issue number4
DOIs
Publication statusPublished - 17 Nov 1995

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology

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