A distal region of the CALC-1 gene is necessary for regulated alternative splicing

D. A. Horn; D. S. Latchman

doi:10.1016/0014-5793(93)81376-B

A distal region of the CALC-1 gene is necessary for regulated alternative splicing

D. A. Horn, D. S. Latchman

Biological Chemistry and Drug Discovery

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

The CALC-1 gene exhibits tissue specific alternative splicing with exons 1-4 being spliced to produce the calcitonin mRNA in thyroid C cells and exons 1-3 and 5-6 being joined to produce the CGRP mRNA in neuronal cells. Previous studies have identified an element in intron 3 within the alternatively spliced region which is critical for this effect to occur. We show here that deletion of sequences downstream of the alternatively spliced region also disrupts the tissue specific pattern of alternative splicing. The manner in which these sequences act is discussed.

Original language	English
Pages (from-to)	123-126
Number of pages	4
Journal	FEBS Letters
Volume	324
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(93)81376-B
Publication status	Published - 14 Jun 1993

Keywords

Alternative splicing
Calcitonin
Calcitonin gene related peptide
Gene regulation

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(93)81376-B

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title = "A distal region of the CALC-1 gene is necessary for regulated alternative splicing",

abstract = "The CALC-1 gene exhibits tissue specific alternative splicing with exons 1-4 being spliced to produce the calcitonin mRNA in thyroid C cells and exons 1-3 and 5-6 being joined to produce the CGRP mRNA in neuronal cells. Previous studies have identified an element in intron 3 within the alternatively spliced region which is critical for this effect to occur. We show here that deletion of sequences downstream of the alternatively spliced region also disrupts the tissue specific pattern of alternative splicing. The manner in which these sequences act is discussed.",

keywords = "Alternative splicing, Calcitonin, Calcitonin gene related peptide, Gene regulation",

author = "Horn, {D. A.} and Latchman, {D. S.}",

note = "Funding Information: Acknowledgements~W e thank Dr. P. Broad for the pRSV-Cal 2 and Cal 3 constructs. DAH was supported by the British Heart Foundation.",

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AU - Horn, D. A.

AU - Latchman, D. S.

N1 - Funding Information: Acknowledgements~W e thank Dr. P. Broad for the pRSV-Cal 2 and Cal 3 constructs. DAH was supported by the British Heart Foundation.

PY - 1993/6/14

Y1 - 1993/6/14

N2 - The CALC-1 gene exhibits tissue specific alternative splicing with exons 1-4 being spliced to produce the calcitonin mRNA in thyroid C cells and exons 1-3 and 5-6 being joined to produce the CGRP mRNA in neuronal cells. Previous studies have identified an element in intron 3 within the alternatively spliced region which is critical for this effect to occur. We show here that deletion of sequences downstream of the alternatively spliced region also disrupts the tissue specific pattern of alternative splicing. The manner in which these sequences act is discussed.

AB - The CALC-1 gene exhibits tissue specific alternative splicing with exons 1-4 being spliced to produce the calcitonin mRNA in thyroid C cells and exons 1-3 and 5-6 being joined to produce the CGRP mRNA in neuronal cells. Previous studies have identified an element in intron 3 within the alternatively spliced region which is critical for this effect to occur. We show here that deletion of sequences downstream of the alternatively spliced region also disrupts the tissue specific pattern of alternative splicing. The manner in which these sequences act is discussed.

KW - Alternative splicing

KW - Calcitonin

KW - Calcitonin gene related peptide

KW - Gene regulation

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DO - 10.1016/0014-5793(93)81376-B

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EP - 126

JO - FEBS Letters

JF - FEBS Letters

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ER -

A distal region of the CALC-1 gene is necessary for regulated alternative splicing

Abstract

Keywords

ASJC Scopus subject areas

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