A fluorophore-conjugated reagent enabling rapid detection, isolation and live tracking of senescent cells

Sophia Magkouta; Dimitris Veroutis; Athanasios Pousias; Angelos Papaspyropoulos; Natassa Pippa; Nikolaos Lougiakis; Konstantinos Kambas; Nefeli Lagopati; Aikaterini Polyzou; Maria Georgiou; Maria Chountoulesi; Stergios Pispas; Spyros Foutadakis; Nicole Pouli; Panagiotis Marakos; Athanassios Kotsinas; Panayotis Verginis; Dimitrios Valakos; Athanasia Mizi; Argyris Papantonis; Giannis Vatsellas; Panagiotis Galanos; Jiri Bartek; Russell Petty; Manuel Serrano; Dimitris Thanos; Charis Roussos; Marco Demaria; Konstantinos Evangelou; Vassilis G. Gorgoulis

doi:10.1016/j.molcel.2023.09.006

A fluorophore-conjugated reagent enabling rapid detection, isolation and live tracking of senescent cells

Sophia Magkouta, Dimitris Veroutis, Athanasios Pousias, Angelos Papaspyropoulos, Natassa Pippa, Nikolaos Lougiakis, Konstantinos Kambas, Nefeli Lagopati, Aikaterini Polyzou, Maria Georgiou, Maria Chountoulesi, Stergios Pispas, Spyros Foutadakis, Nicole Pouli, Panagiotis Marakos, Athanassios Kotsinas, Panayotis Verginis, Dimitrios Valakos, Athanasia Mizi, Argyris PapantonisGiannis Vatsellas, Panagiotis Galanos, Jiri Bartek, Russell Petty, Manuel Serrano, Dimitris Thanos, Charis Roussos, Marco Demaria, Konstantinos Evangelou, Vassilis G. Gorgoulis (Lead / Corresponding author)

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Cellular senescence is a stress-response mechanism implicated in various physiological processes, diseases, and aging. Current detection approaches have partially addressed the issue of senescent cell identification in clinical specimens. Effective methodologies enabling precise isolation or live tracking of senescent cells are still lacking. In-depth analysis of truly senescent cells is, therefore, an extremely challenging task. We report (1) the synthesis and validation of a fluorophore-conjugated, Sudan Black-B analog (GLF16), suitable for in vivo and in vitro analysis of senescence by fluorescence microscopy and flow cytometry and (2) the development and application of a GLF16-carrying micelle vector facilitating GLF16 uptake by living senescent cells in vivo and in vitro. The compound and the applied methodology render isolation of senescent cells an easy, rapid, and precise process. Straightforward nanocarrier-mediated GLF16 delivery in live senescent cells comprises a unique tool for characterization of senescence at an unprecedented depth.

Original language	English
Pages (from-to)	3558-3573.e7
Number of pages	24
Journal	Molecular Cell
Volume	83
Issue number	19
DOIs	https://doi.org/10.1016/j.molcel.2023.09.006
Publication status	Published - 5 Oct 2023

Keywords

Indicators and Reagents
Cellular Senescence
Flow Cytometry
senescence
micelle
live tracking
GLF-16
flow cytometry
fluorescence microscopy
optimized SBB analogs

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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Author Accepted ManuscriptAccepted author manuscript, 7.04 MBLicence: CC BY-NC-ND

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Magkouta, S., Veroutis, D., Pousias, A., Papaspyropoulos, A., Pippa, N., Lougiakis, N., Kambas, K., Lagopati, N., Polyzou, A., Georgiou, M., Chountoulesi, M., Pispas, S., Foutadakis, S., Pouli, N., Marakos, P., Kotsinas, A., Verginis, P., Valakos, D., Mizi, A., ... Gorgoulis, V. G. (2023). A fluorophore-conjugated reagent enabling rapid detection, isolation and live tracking of senescent cells. Molecular Cell, 83(19), 3558-3573.e7. https://doi.org/10.1016/j.molcel.2023.09.006

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title = "A fluorophore-conjugated reagent enabling rapid detection, isolation and live tracking of senescent cells",

abstract = "Cellular senescence is a stress-response mechanism implicated in various physiological processes, diseases, and aging. Current detection approaches have partially addressed the issue of senescent cell identification in clinical specimens. Effective methodologies enabling precise isolation or live tracking of senescent cells are still lacking. In-depth analysis of truly senescent cells is, therefore, an extremely challenging task. We report (1) the synthesis and validation of a fluorophore-conjugated, Sudan Black-B analog (GLF16), suitable for in vivo and in vitro analysis of senescence by fluorescence microscopy and flow cytometry and (2) the development and application of a GLF16-carrying micelle vector facilitating GLF16 uptake by living senescent cells in vivo and in vitro. The compound and the applied methodology render isolation of senescent cells an easy, rapid, and precise process. Straightforward nanocarrier-mediated GLF16 delivery in live senescent cells comprises a unique tool for characterization of senescence at an unprecedented depth.",

keywords = "Indicators and Reagents, Cellular Senescence, Flow Cytometry, senescence, micelle, live tracking, GLF-16, flow cytometry, fluorescence microscopy, optimized SBB analogs",

author = "Sophia Magkouta and Dimitris Veroutis and Athanasios Pousias and Angelos Papaspyropoulos and Natassa Pippa and Nikolaos Lougiakis and Konstantinos Kambas and Nefeli Lagopati and Aikaterini Polyzou and Maria Georgiou and Maria Chountoulesi and Stergios Pispas and Spyros Foutadakis and Nicole Pouli and Panagiotis Marakos and Athanassios Kotsinas and Panayotis Verginis and Dimitrios Valakos and Athanasia Mizi and Argyris Papantonis and Giannis Vatsellas and Panagiotis Galanos and Jiri Bartek and Russell Petty and Manuel Serrano and Dimitris Thanos and Charis Roussos and Marco Demaria and Konstantinos Evangelou and Gorgoulis, {Vassilis G.}",

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Magkouta, S, Veroutis, D, Pousias, A, Papaspyropoulos, A, Pippa, N, Lougiakis, N, Kambas, K, Lagopati, N, Polyzou, A, Georgiou, M, Chountoulesi, M, Pispas, S, Foutadakis, S, Pouli, N, Marakos, P, Kotsinas, A, Verginis, P, Valakos, D, Mizi, A, Papantonis, A, Vatsellas, G, Galanos, P, Bartek, J, Petty, R, Serrano, M, Thanos, D, Roussos, C, Demaria, M, Evangelou, K & Gorgoulis, VG 2023, 'A fluorophore-conjugated reagent enabling rapid detection, isolation and live tracking of senescent cells', Molecular Cell, vol. 83, no. 19, pp. 3558-3573.e7. https://doi.org/10.1016/j.molcel.2023.09.006

TY - JOUR

T1 - A fluorophore-conjugated reagent enabling rapid detection, isolation and live tracking of senescent cells

AU - Magkouta, Sophia

AU - Veroutis, Dimitris

AU - Pousias, Athanasios

AU - Papaspyropoulos, Angelos

AU - Pippa, Natassa

AU - Lougiakis, Nikolaos

AU - Kambas, Konstantinos

AU - Lagopati, Nefeli

AU - Polyzou, Aikaterini

AU - Georgiou, Maria

AU - Chountoulesi, Maria

AU - Pispas, Stergios

AU - Foutadakis, Spyros

AU - Pouli, Nicole

AU - Marakos, Panagiotis

AU - Kotsinas, Athanassios

AU - Verginis, Panayotis

AU - Valakos, Dimitrios

AU - Mizi, Athanasia

AU - Papantonis, Argyris

AU - Vatsellas, Giannis

AU - Galanos, Panagiotis

AU - Bartek, Jiri

AU - Petty, Russell

AU - Serrano, Manuel

AU - Thanos, Dimitris

AU - Roussos, Charis

AU - Demaria, Marco

AU - Evangelou, Konstantinos

AU - Gorgoulis, Vassilis G.

PY - 2023/10/5

Y1 - 2023/10/5

N2 - Cellular senescence is a stress-response mechanism implicated in various physiological processes, diseases, and aging. Current detection approaches have partially addressed the issue of senescent cell identification in clinical specimens. Effective methodologies enabling precise isolation or live tracking of senescent cells are still lacking. In-depth analysis of truly senescent cells is, therefore, an extremely challenging task. We report (1) the synthesis and validation of a fluorophore-conjugated, Sudan Black-B analog (GLF16), suitable for in vivo and in vitro analysis of senescence by fluorescence microscopy and flow cytometry and (2) the development and application of a GLF16-carrying micelle vector facilitating GLF16 uptake by living senescent cells in vivo and in vitro. The compound and the applied methodology render isolation of senescent cells an easy, rapid, and precise process. Straightforward nanocarrier-mediated GLF16 delivery in live senescent cells comprises a unique tool for characterization of senescence at an unprecedented depth.

AB - Cellular senescence is a stress-response mechanism implicated in various physiological processes, diseases, and aging. Current detection approaches have partially addressed the issue of senescent cell identification in clinical specimens. Effective methodologies enabling precise isolation or live tracking of senescent cells are still lacking. In-depth analysis of truly senescent cells is, therefore, an extremely challenging task. We report (1) the synthesis and validation of a fluorophore-conjugated, Sudan Black-B analog (GLF16), suitable for in vivo and in vitro analysis of senescence by fluorescence microscopy and flow cytometry and (2) the development and application of a GLF16-carrying micelle vector facilitating GLF16 uptake by living senescent cells in vivo and in vitro. The compound and the applied methodology render isolation of senescent cells an easy, rapid, and precise process. Straightforward nanocarrier-mediated GLF16 delivery in live senescent cells comprises a unique tool for characterization of senescence at an unprecedented depth.

KW - Indicators and Reagents

KW - Cellular Senescence

KW - Flow Cytometry

KW - senescence

KW - micelle

KW - live tracking

KW - GLF-16

KW - flow cytometry

KW - fluorescence microscopy

KW - optimized SBB analogs

U2 - 10.1016/j.molcel.2023.09.006

DO - 10.1016/j.molcel.2023.09.006

M3 - Article

C2 - 37802028

SN - 1097-2765

VL - 83

SP - 3558-3573.e7

JO - Molecular Cell

JF - Molecular Cell

IS - 19

ER -

A fluorophore-conjugated reagent enabling rapid detection, isolation and live tracking of senescent cells

Abstract

Keywords

ASJC Scopus subject areas

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