A genome sequence assembly of the phototactic and optogenetic model fungus Blastocladiella emersonii reveals a diversified nucleotide-cyclase repertoire

Guy Leonard, Luis Javier Galindo, David S. Milner, Gabriela Mol Avelar, André L. Gomes-Vieira, Suely L. Gomes, Thomas A. Richards (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)
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Abstract

The chytrid fungus Blastocladiella emersonii produces spores with swimming tails (zoospores); these cells can sense and swim toward light. Interest in this species stems from ongoing efforts to develop B. emersonii as a model for understanding the evolution of phototaxis and the molecular cell biology of the associated optogenetic circuits. Here, we report a highly contiguous genome assembly and gene annotation of the B. emersonii American Type Culture Collection 22665 strain. We integrate a PacBio long-read library with an Illumina paired-end genomic sequence survey leading to an assembly of 21 contigs totaling 34.27 Mb. Using these data, we assess the diversity of sensory system encoding genes. These analyses identify a rich complement of G-protein-coupled receptors, ion transporters, and nucleotide cyclases, all of which have been diversified by domain recombination and tandem duplication. In many cases, these domain combinations have led to the fusion of a protein domain to a transmembrane domain, tying a putative signaling function to the cell membrane. This pattern is consistent with the diversification of the B. emersonii sensory-signaling systems, which likely plays a varied role in the complex life cycle of this fungus.

Original languageEnglish
Article numberevac157
Number of pages9
JournalGenome Biology and Evolution
Volume14
Issue number12
Early online date25 Oct 2022
DOIs
Publication statusPublished - 8 Dec 2022

Keywords

  • chytrid
  • fungi
  • light perception
  • sensory system

ASJC Scopus subject areas

  • General Medicine

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