A genome sequence assembly of the phototactic and optogenetic model fungus Blastocladiella emersonii reveals a diversified nucleotide-cyclase repertoire

Guy Leonard, Luis Javier Galindo, David S. Milner, Gabriela Mol Avelar, André L. Gomes-Vieira, Suely L. Gomes, Thomas A. Richards (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

Abstract

The chytrid fungus Blastocladiella emersonii produces spores with swimming tails (zoospores); these cells can sense and swim towards light. Interest in this species stems from ongoing efforts to develop B. emersonii as a model for understanding the evolution of phototaxis and the molecular cell biology of the associated optogenetic circuits. Here we report a highly-contiguous genome assembly and gene annotation of the B. emersonii ATCC 22665 strain. We integrate a PacBio long-read library with an Illumina paired-end genomic sequence survey leading to an assembly of 21 contigs totalling 34.27 Mb. Using these data, we assess the diversity of sensory system encoding genes. These analyses identify a rich complement of G protein-coupled receptors (GPCRs), ion transporters and nucleotide-cyclases, all of which had been diversified by domain recombination and tandem duplication. In many cases these domain combinations have led to the fusion of a protein domain to a transmembrane domain, tying a putative signalling function to the cell membrane. This pattern is consistent with the diversification of the B. emersonii sensory-signalling systems, which likely plays a varied role in the complex lifecycle of this fungus.

Original languageEnglish
Article numberevac157
JournalGenome Biology and Evolution
Early online date25 Oct 2022
DOIs
Publication statusE-pub ahead of print - 25 Oct 2022

Keywords

  • chytrid
  • Fungi
  • Sensory system
  • Light perception

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