A genome wide association scan for (1,3;1,4)-β-glucan content in the grain of contemporary 2-row Spring and Winter barleys

Kelly Houston, Joanne Russell, Miriam Schreiber, Claire Halpin, Helena Oakey, Jennifer M. Washington, Allan Booth, Neil Shirley, Rachel A. Burton, Geoffrey B. Fincher, Robbie Waugh (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    32 Citations (Scopus)
    156 Downloads (Pure)

    Abstract

    Background: (1,3;1,4)-β-Glucan is an important component of the cell walls of barley grain as it affects processability during the production of alcoholic beverages and has significant human health benefits when consumed aboverecommended threshold levels. This leads to diametrically opposed quality requirements for different applications as low levels of (1,3;1,4)-β-glucan are required for brewing and distilling and high levels for positive impacts on humanhealth.
    Results: We quantified grain (1,3;1,4)-β-glucan content in a collection of 399 2-row Spring-type, and 204 2-row Winter-type elite barley cultivars originating mainly from north western Europe. We combined these data with genotypic information derived using a 9 K Illumina iSelect SNP platform and subsequently carried out a Genome Wide Association Scan (GWAS). Statistical analysis accounting for residual genetic structure within the germplasm collection allowed us to identify significant associations between molecular markers and the phenotypic data. By anchoring the regions that contain these associations to the barley genome assembly we catalogued genes underlying the associations. Based on gene annotations and transcript abundance data we identified candidate genes.
    Conclusions: We show that a region of the genome on chromosome 2 containing a cluster of CELLULOSE SYNTHASE-LIKE (Csl) genes, including CslF3, CslF4, CslF8, CslF10, CslF12 and CslH, as well as a region on chromosome 1H containingCslF9, are associated with the phenotype in this germplasm. We also observed that several regions identified by GWAS contain glycoside hydrolases that are possibly involved in (1,3;1,4)-β-glucan breakdown, together with other genes that might participate in (1,3;1,4)-β-glucan synthesis, re-modelling or regulation. This analysis provides new opportunities for understanding the genes related to the regulation of (1,3;1,4)-β-glucan content in cereal grains.
    Original languageEnglish
    Article number907
    Number of pages15
    JournalBMC Genomics
    Volume15
    DOIs
    Publication statusPublished - 17 Oct 2014

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  • Student Theses

    Identification of genes involved in (1,3;1,4)-β-glucan synthesis in barley (Hordeum vulgare)

    Author: Schreiber, M., 2016

    Supervisor: Halpin, C. (Supervisor) & Waugh, R. (Supervisor)

    Student thesis: Doctoral ThesisDoctor of Philosophy

    Cite this

    Houston, K., Russell, J., Schreiber, M., Halpin, C., Oakey, H., Washington, J. M., Booth, A., Shirley, N., Burton, R. A., Fincher, G. B., & Waugh, R. (2014). A genome wide association scan for (1,3;1,4)-β-glucan content in the grain of contemporary 2-row Spring and Winter barleys. BMC Genomics, 15, [907]. https://doi.org/10.1186/1471-2164-15-907