TY - JOUR
T1 - A histochemical study of carbonic anhydrase in the plasma membranes of human oral epithelial cells
AU - Christie, K.N.
AU - Thomson, C.
AU - Ogden, G. R.
AU - Hopwood, D.
PY - 1995
Y1 - 1995
N2 - Carbonic anhydrase (EC 4.2.1.1) was detected histochemically from the following regions in patients of various ages (14-84 yr): buccal mucosa, buccal flap, hard palate and tongue. The enzyme was principally located in the cell membranes but was also present in nuclei. There was a gradation in activity from basal (strong) to superficial cells (weak/negative). The carbonic anhydrase inhibitors ethoxyzolamide and acetazolamide abolished activity at 0.001 mM, but were ineffective, even at 1.2 mM, against a reaction associated with the granules of the stratum granulosum. No activity was detected in the absence of bicarbonate from the substrate.
AB - Carbonic anhydrase (EC 4.2.1.1) was detected histochemically from the following regions in patients of various ages (14-84 yr): buccal mucosa, buccal flap, hard palate and tongue. The enzyme was principally located in the cell membranes but was also present in nuclei. There was a gradation in activity from basal (strong) to superficial cells (weak/negative). The carbonic anhydrase inhibitors ethoxyzolamide and acetazolamide abolished activity at 0.001 mM, but were ineffective, even at 1.2 mM, against a reaction associated with the granules of the stratum granulosum. No activity was detected in the absence of bicarbonate from the substrate.
UR - http://www.scopus.com/inward/record.url?scp=0029299055&partnerID=8YFLogxK
U2 - 10.1016/0003-9969(94)00193-F
DO - 10.1016/0003-9969(94)00193-F
M3 - Article
AN - SCOPUS:0029299055
SN - 0003-9969
VL - 40
SP - 447
EP - 451
JO - Archives of Oral Biology
JF - Archives of Oral Biology
IS - 5
ER -