A histochemical study of carbonic anhydrase in the plasma membranes of human oral epithelial cells

K.N. Christie, C. Thomson, G. R. Ogden, D. Hopwood

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Carbonic anhydrase (EC 4.2.1.1) was detected histochemically from the following regions in patients of various ages (14-84 yr): buccal mucosa, buccal flap, hard palate and tongue. The enzyme was principally located in the cell membranes but was also present in nuclei. There was a gradation in activity from basal (strong) to superficial cells (weak/negative). The carbonic anhydrase inhibitors ethoxyzolamide and acetazolamide abolished activity at 0.001 mM, but were ineffective, even at 1.2 mM, against a reaction associated with the granules of the stratum granulosum. No activity was detected in the absence of bicarbonate from the substrate.
    Original languageEnglish
    Pages (from-to)447-451
    Number of pages5
    JournalArchives of Oral Biology
    Volume40
    Issue number5
    DOIs
    Publication statusPublished - 1995

    Fingerprint Dive into the research topics of 'A histochemical study of carbonic anhydrase in the plasma membranes of human oral epithelial cells'. Together they form a unique fingerprint.

  • Cite this