A histochemical study of carbonic anhydrase in the plasma membranes of human oral epithelial cells

TY - JOUR

T1 - A histochemical study of carbonic anhydrase in the plasma membranes of human oral epithelial cells

AU - Christie, K.N.

AU - Thomson, C.

AU - Ogden, G. R.

AU - Hopwood, D.

PY - 1995

Y1 - 1995

N2 - Carbonic anhydrase (EC 4.2.1.1) was detected histochemically from the following regions in patients of various ages (14-84 yr): buccal mucosa, buccal flap, hard palate and tongue. The enzyme was principally located in the cell membranes but was also present in nuclei. There was a gradation in activity from basal (strong) to superficial cells (weak/negative). The carbonic anhydrase inhibitors ethoxyzolamide and acetazolamide abolished activity at 0.001 mM, but were ineffective, even at 1.2 mM, against a reaction associated with the granules of the stratum granulosum. No activity was detected in the absence of bicarbonate from the substrate.

AB - Carbonic anhydrase (EC 4.2.1.1) was detected histochemically from the following regions in patients of various ages (14-84 yr): buccal mucosa, buccal flap, hard palate and tongue. The enzyme was principally located in the cell membranes but was also present in nuclei. There was a gradation in activity from basal (strong) to superficial cells (weak/negative). The carbonic anhydrase inhibitors ethoxyzolamide and acetazolamide abolished activity at 0.001 mM, but were ineffective, even at 1.2 mM, against a reaction associated with the granules of the stratum granulosum. No activity was detected in the absence of bicarbonate from the substrate.

UR - https://www.scopus.com/pages/publications/0029299055

U2 - 10.1016/0003-9969(94)00193-F

DO - 10.1016/0003-9969(94)00193-F

M3 - Article

AN - SCOPUS:0029299055

SN - 0003-9969

VL - 40

SP - 447

EP - 451

JO - Archives of Oral Biology

JF - Archives of Oral Biology

IS - 5

ER -