Projects per year
Abstract
The nuclear lamina supports many functions, including maintaining nuclear structure and gene expression control, and correct spatiotemporal assembly is vital to meet these activities. Recently, multiple lamina systems have been described that, despite independent evolutionary origins, share analogous functions. In trypanosomatids the two known lamina proteins, NUP-1 and NUP-2, have molecular masses of 450 and 170 kDa, respectively, which demands a distinct architecture from the ∼60 kDa lamin-based system of metazoa and other lineages. To uncover organizational principles for the trypanosome laminawe generated NUP-1 deletion mutants to identify domains and their arrangements responsible for oligomerization. We found that both the N- and C-termini act as interaction hubs, and that perturbation of these interactions impacts additional components of the lamina and nuclear envelope. Furthermore, the assembly of NUP- 1 terminal domains suggests intrinsic organizational capacity. Remarkably, there is little impact on silencing of telomeric variant surface glycoprotein genes. We suggest that both terminal domains of NUP-1 have roles in assembling the trypanosome lamina and propose a novel architecture based on a hub-and-spoke configuration.
Original language | English |
---|---|
Article number | jcs251264 |
Number of pages | 16 |
Journal | Journal of Cell Science |
Volume | 134 |
Issue number | 12 |
Early online date | 19 May 2021 |
DOIs | |
Publication status | Published - 21 Jun 2021 |
Keywords
- lamina
- macromolecular assembly
- trypanosomatids
- nuclear organisation
- heterochromatin
- Heterochromatin
- Trypanosomatid
- Nuclear organization
- Macromolecular assembly
- Lamina
ASJC Scopus subject areas
- Cell Biology
Fingerprint
Dive into the research topics of 'A hub and spoke nuclear lamina architecture in trypanosomes'. Together they form a unique fingerprint.Projects
- 2 Finished
-
A Systems Approach for Understanding Cell Surface Dynamics in Trypanosomes (Investigator Award)
Field, M. (Investigator)
1/10/17 → 31/03/24
Project: Research
-
Control of Gene Expression in Trypanosoes: Defining the Nuclear Lamina
Field, M. (Investigator)
1/07/16 → 29/02/20
Project: Research