A Phytophthora infestans RXLR effector targets plant PP1c isoforms that promote late blight disease

Petra C. Boevink; Xiaodan Wang; Hazel McLellan; Qin He; Shaista Naqvi; Miles R. Armstrong; Wei Zhang; Ingo Hein; Eleanor M. Gilroy; Zhendong Tian; Paul R. J. Birch

doi:10.1038/ncomms10311

A Phytophthora infestans RXLR effector targets plant PP1c isoforms that promote late blight disease

Petra C. Boevink, Xiaodan Wang, Hazel McLellan, Qin He, Shaista Naqvi, Miles R. Armstrong, Wei Zhang, Ingo Hein, Eleanor M. Gilroy, Zhendong Tian, Paul R. J. Birch (Lead / Corresponding author)

Plant Sciences

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115 Citations (Scopus)

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Abstract

Plant pathogens deliver effectors to alter host processes. Knowledge of how effectors target and manipulate host proteins is critical to understand crop disease. Here we show that in planta expression of the RXLR effector Pi04314 enhances leaf colonisation by Phytophthora infestans via activity in the host nucleus and attenuates induction of jasmonic and salicylic acid responsive
genes. Pi04314 interacts with three host protein phosphatase 1 catalytic (PP1c) isoforms, causing their re-localization from the nucleolus to the nucleoplasm. Re-localisation of PP1c-1 also occurs during infection and is dependent on an R/KVxF motif in the effector. Silencing the PP1c isoforms or overexpression of a phosphatase-dead PP1c-1 mutant attenuates infection, demonstrating that host PP1c activity is required for disease. Moreover, expression of PP1c-1mut abolishes enhanced leaf colonisation mediated by in planta Pi04314 expression. We argue that PP1c isoforms are susceptibility factors forming holoenzymes with Pi04314 to promote late blight disease.

Original language	English
Article number	10311
Journal	Nature Communications
Volume	7
DOIs	https://doi.org/10.1038/ncomms10311
Publication status	Published - 29 Jan 2016

Keywords

Biological sciences
Plant sciences
Microbiology

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Undermining Effector-Targeted Susceptibility Factors to Provide Late Blight Resistance (Industrial Partnership award)
Birch, P. (Investigator)
Biotechnology and Biological Sciences Research Council
1/04/16 → 31/10/19
Project: Research
UK-China Partnership to Develop Durable Late Blight Disease Resistance in Potato (Partnering Award)
Birch, P. (Investigator)
Biotechnology and Biological Sciences Research Council
1/05/14 → 30/04/17
Project: Research
The Contribution of Phytophthora Effectors to Host Range and Non-Host Resistance (Joint with James Hutton Institute and University of Warwick and Industrial Partners)
Birch, P. (Investigator) & Huitema, E. (Investigator)
Biotechnology and Biological Sciences Research Council
1/08/13 → 12/04/17
Project: Research

Birch, Paul
- Plant Sciences - Professor of Plant Pathology
Person: Academic

Cite this

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title = "A Phytophthora infestans RXLR effector targets plant PP1c isoforms that promote late blight disease",

abstract = "Plant pathogens deliver effectors to alter host processes. Knowledge of how effectors target and manipulate host proteins is critical to understand crop disease. Here we show that in planta expression of the RXLR effector Pi04314 enhances leaf colonisation by Phytophthora infestans via activity in the host nucleus and attenuates induction of jasmonic and salicylic acid responsivegenes. Pi04314 interacts with three host protein phosphatase 1 catalytic (PP1c) isoforms, causing their re-localization from the nucleolus to the nucleoplasm. Re-localisation of PP1c-1 also occurs during infection and is dependent on an R/KVxF motif in the effector. Silencing the PP1c isoforms or overexpression of a phosphatase-dead PP1c-1 mutant attenuates infection, demonstrating that host PP1c activity is required for disease. Moreover, expression of PP1c-1mut abolishes enhanced leaf colonisation mediated by in planta Pi04314 expression. We argue that PP1c isoforms are susceptibility factors forming holoenzymes with Pi04314 to promote late blight disease.",

keywords = "Biological sciences, Plant sciences, Microbiology",

author = "Boevink, {Petra C.} and Xiaodan Wang and Hazel McLellan and Qin He and Shaista Naqvi and Armstrong, {Miles R.} and Wei Zhang and Ingo Hein and Gilroy, {Eleanor M.} and Zhendong Tian and Birch, {Paul R. J.}",

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language = "English",

volume = "7",

journal = "Nature Communications",

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T1 - A Phytophthora infestans RXLR effector targets plant PP1c isoforms that promote late blight disease

AU - Boevink, Petra C.

AU - Wang, Xiaodan

AU - McLellan, Hazel

AU - He, Qin

AU - Naqvi, Shaista

AU - Armstrong, Miles R.

AU - Zhang, Wei

AU - Hein, Ingo

AU - Gilroy, Eleanor M.

AU - Tian, Zhendong

AU - Birch, Paul R. J.

PY - 2016/1/29

Y1 - 2016/1/29

N2 - Plant pathogens deliver effectors to alter host processes. Knowledge of how effectors target and manipulate host proteins is critical to understand crop disease. Here we show that in planta expression of the RXLR effector Pi04314 enhances leaf colonisation by Phytophthora infestans via activity in the host nucleus and attenuates induction of jasmonic and salicylic acid responsivegenes. Pi04314 interacts with three host protein phosphatase 1 catalytic (PP1c) isoforms, causing their re-localization from the nucleolus to the nucleoplasm. Re-localisation of PP1c-1 also occurs during infection and is dependent on an R/KVxF motif in the effector. Silencing the PP1c isoforms or overexpression of a phosphatase-dead PP1c-1 mutant attenuates infection, demonstrating that host PP1c activity is required for disease. Moreover, expression of PP1c-1mut abolishes enhanced leaf colonisation mediated by in planta Pi04314 expression. We argue that PP1c isoforms are susceptibility factors forming holoenzymes with Pi04314 to promote late blight disease.

AB - Plant pathogens deliver effectors to alter host processes. Knowledge of how effectors target and manipulate host proteins is critical to understand crop disease. Here we show that in planta expression of the RXLR effector Pi04314 enhances leaf colonisation by Phytophthora infestans via activity in the host nucleus and attenuates induction of jasmonic and salicylic acid responsivegenes. Pi04314 interacts with three host protein phosphatase 1 catalytic (PP1c) isoforms, causing their re-localization from the nucleolus to the nucleoplasm. Re-localisation of PP1c-1 also occurs during infection and is dependent on an R/KVxF motif in the effector. Silencing the PP1c isoforms or overexpression of a phosphatase-dead PP1c-1 mutant attenuates infection, demonstrating that host PP1c activity is required for disease. Moreover, expression of PP1c-1mut abolishes enhanced leaf colonisation mediated by in planta Pi04314 expression. We argue that PP1c isoforms are susceptibility factors forming holoenzymes with Pi04314 to promote late blight disease.

KW - Biological sciences

KW - Plant sciences

KW - Microbiology

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DO - 10.1038/ncomms10311

M3 - Article

C2 - 26822079

SN - 2041-1723

VL - 7

JO - Nature Communications

JF - Nature Communications

M1 - 10311

ER -

A Phytophthora infestans RXLR effector targets plant PP1c isoforms that promote late blight disease

Abstract

Keywords

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Projects

Undermining Effector-Targeted Susceptibility Factors to Provide Late Blight Resistance (Industrial Partnership award)

UK-China Partnership to Develop Durable Late Blight Disease Resistance in Potato (Partnering Award)

The Contribution of Phytophthora Effectors to Host Range and Non-Host Resistance (Joint with James Hutton Institute and University of Warwick and Industrial Partners)

Profiles

Birch, Paul

Cite this