A Phytophthora infestans RXLR effector targets plant PP1c isoforms that promote late blight disease

Petra C. Boevink, Xiaodan Wang, Hazel McLellan, Qin He, Shaista Naqvi, Miles R. Armstrong, Wei Zhang, Ingo Hein, Eleanor M. Gilroy, Zhendong Tian, Paul R. J. Birch (Lead / Corresponding author)

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Abstract

Plant pathogens deliver effectors to alter host processes. Knowledge of how effectors target and manipulate host proteins is critical to understand crop disease. Here we show that in planta expression of the RXLR effector Pi04314 enhances leaf colonisation by Phytophthora infestans via activity in the host nucleus and attenuates induction of jasmonic and salicylic acid responsive
genes. Pi04314 interacts with three host protein phosphatase 1 catalytic (PP1c) isoforms, causing their re-localization from the nucleolus to the nucleoplasm. Re-localisation of PP1c-1 also occurs during infection and is dependent on an R/KVxF motif in the effector. Silencing the PP1c isoforms or overexpression of a phosphatase-dead PP1c-1 mutant attenuates infection, demonstrating that host PP1c activity is required for disease. Moreover, expression of PP1c-1mut abolishes enhanced leaf colonisation mediated by in planta Pi04314 expression. We argue that PP1c isoforms are susceptibility factors forming holoenzymes with Pi04314 to promote late blight disease.
Original languageEnglish
Article number10311
JournalNature Communications
Volume7
DOIs
Publication statusPublished - 29 Jan 2016

Fingerprint

blight
Phytophthora infestans
effectors
Protein Phosphatase 1
Plant Proteins
phosphatases
Protein Isoforms
proteins
infectious diseases
leaves
Holoenzymes
Salicylic Acid
Pathogens
pathogens
Infection
Phosphoric Monoester Hydrolases
crops
Crops
Catalyst activity
catalytic activity

Keywords

  • Biological sciences
  • Plant sciences
  • Microbiology

Cite this

Boevink, Petra C. ; Wang, Xiaodan ; McLellan, Hazel ; He, Qin ; Naqvi, Shaista ; Armstrong, Miles R. ; Zhang, Wei ; Hein, Ingo ; Gilroy, Eleanor M. ; Tian, Zhendong ; Birch, Paul R. J. / A Phytophthora infestans RXLR effector targets plant PP1c isoforms that promote late blight disease. In: Nature Communications. 2016 ; Vol. 7.
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A Phytophthora infestans RXLR effector targets plant PP1c isoforms that promote late blight disease. / Boevink, Petra C.; Wang, Xiaodan; McLellan, Hazel; He, Qin; Naqvi, Shaista; Armstrong, Miles R.; Zhang, Wei; Hein, Ingo; Gilroy, Eleanor M.; Tian, Zhendong; Birch, Paul R. J. (Lead / Corresponding author).

In: Nature Communications, Vol. 7, 10311, 29.01.2016.

Research output: Contribution to journalArticle

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AU - Boevink, Petra C.

AU - Wang, Xiaodan

AU - McLellan, Hazel

AU - He, Qin

AU - Naqvi, Shaista

AU - Armstrong, Miles R.

AU - Zhang, Wei

AU - Hein, Ingo

AU - Gilroy, Eleanor M.

AU - Tian, Zhendong

AU - Birch, Paul R. J.

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N2 - Plant pathogens deliver effectors to alter host processes. Knowledge of how effectors target and manipulate host proteins is critical to understand crop disease. Here we show that in planta expression of the RXLR effector Pi04314 enhances leaf colonisation by Phytophthora infestans via activity in the host nucleus and attenuates induction of jasmonic and salicylic acid responsivegenes. Pi04314 interacts with three host protein phosphatase 1 catalytic (PP1c) isoforms, causing their re-localization from the nucleolus to the nucleoplasm. Re-localisation of PP1c-1 also occurs during infection and is dependent on an R/KVxF motif in the effector. Silencing the PP1c isoforms or overexpression of a phosphatase-dead PP1c-1 mutant attenuates infection, demonstrating that host PP1c activity is required for disease. Moreover, expression of PP1c-1mut abolishes enhanced leaf colonisation mediated by in planta Pi04314 expression. We argue that PP1c isoforms are susceptibility factors forming holoenzymes with Pi04314 to promote late blight disease.

AB - Plant pathogens deliver effectors to alter host processes. Knowledge of how effectors target and manipulate host proteins is critical to understand crop disease. Here we show that in planta expression of the RXLR effector Pi04314 enhances leaf colonisation by Phytophthora infestans via activity in the host nucleus and attenuates induction of jasmonic and salicylic acid responsivegenes. Pi04314 interacts with three host protein phosphatase 1 catalytic (PP1c) isoforms, causing their re-localization from the nucleolus to the nucleoplasm. Re-localisation of PP1c-1 also occurs during infection and is dependent on an R/KVxF motif in the effector. Silencing the PP1c isoforms or overexpression of a phosphatase-dead PP1c-1 mutant attenuates infection, demonstrating that host PP1c activity is required for disease. Moreover, expression of PP1c-1mut abolishes enhanced leaf colonisation mediated by in planta Pi04314 expression. We argue that PP1c isoforms are susceptibility factors forming holoenzymes with Pi04314 to promote late blight disease.

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