A knowledge-based architecture for protein sequence analysis and structure prediction

TY - JOUR

T1 - A knowledge-based architecture for protein sequence analysis and structure prediction

AU - Clark, D A

AU - Barton, G J

AU - Rawlings, C J

PY - 1990/6

Y1 - 1990/6

N2 - Methods for analyzing the amino-acid sequence of a protein for the purposes of predicting its three-dimensional structure were systematically analyzed using knowledge engineering techniques. The resulting entities (data) and relations (processing methods and constraints) have been represented within a generalized dependency network consisting of 29 nodes and over 100 links. It is argued that such a representation meets the requirements of knowledge-based systems in molecular biology. This network is used as the architecture for a prototype knowledge-based system that simulates logically the processes used in protein structure prediction. Although developed specifically for applications in protein structure prediction, the network architecture provides a strategy for tackling the general problem of orchestrating and integrating the diverse sources of knowledge that are characteristic of many areas of science.

AB - Methods for analyzing the amino-acid sequence of a protein for the purposes of predicting its three-dimensional structure were systematically analyzed using knowledge engineering techniques. The resulting entities (data) and relations (processing methods and constraints) have been represented within a generalized dependency network consisting of 29 nodes and over 100 links. It is argued that such a representation meets the requirements of knowledge-based systems in molecular biology. This network is used as the architecture for a prototype knowledge-based system that simulates logically the processes used in protein structure prediction. Although developed specifically for applications in protein structure prediction, the network architecture provides a strategy for tackling the general problem of orchestrating and integrating the diverse sources of knowledge that are characteristic of many areas of science.

KW - Amino Acid Sequence

KW - Artificial Intelligence

KW - Protein Conformation

KW - Proteins

KW - Tryptophan Synthase

U2 - 10.1016/0263-7855(90)80089-X

DO - 10.1016/0263-7855(90)80089-X

M3 - Article

C2 - 2282357

SN - 0263-7855

VL - 8

SP - 94

EP - 107

JO - Journal of Molecular Graphics

JF - Journal of Molecular Graphics

IS - 2

ER -