A microarray-based high throughput molecular marker genotyping method: the tagged microarray marker (TAM) approach

Andrew J. Flavell, Viacheslav N. Bolshakov, Allan Booth, Runchun Jing, Joanne Russell, T. H. Noel Ellis, Peter Isaac

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    52 Citations (Scopus)

    Abstract

    A microarray-based method has been developed for scoring thousands of DNAs for a co-dominant molecular marker on a glass slide. The approach was developed to detect insertional polymorphism of transposons and works well with single nucleotide polymorphism (SNP) markers. Biotinterminated allele-specific PCR products are spotted unpurified onto streptavidin-coated glass slides and visualised by hybridisation of fluorescent detector oligonucleotides to tags attached to the allelespecific PCR primers. Two tagged primer oligonucleotides are used per locus and each tag is detected by hybridisation to a concatameric DNA probe labelled with multiple fluorochromes.
    Original languageEnglish
    Pages (from-to)e115
    JournalNucleic Acids Research
    Volume31
    Issue number19
    DOIs
    Publication statusPublished - Oct 2003

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