A microarray-based high throughput molecular marker genotyping method: the tagged microarray marker (TAM) approach

Andrew J. Flavell; Viacheslav N. Bolshakov; Allan Booth; Runchun Jing; Joanne Russell; T. H. Noel Ellis; Peter Isaac

doi:10.1093/nar/gng113

A microarray-based high throughput molecular marker genotyping method: the tagged microarray marker (TAM) approach

Andrew J. Flavell, Viacheslav N. Bolshakov, Allan Booth, Runchun Jing, Joanne Russell, T. H. Noel Ellis, Peter Isaac

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Abstract

A microarray-based method has been developed for scoring thousands of DNAs for a co-dominant molecular marker on a glass slide. The approach was developed to detect insertional polymorphism of transposons and works well with single nucleotide polymorphism (SNP) markers. Biotinterminated allele-specific PCR products are spotted unpurified onto streptavidin-coated glass slides and visualised by hybridisation of fluorescent detector oligonucleotides to tags attached to the allelespecific PCR primers. Two tagged primer oligonucleotides are used per locus and each tag is detected by hybridisation to a concatameric DNA probe labelled with multiple fluorochromes.

Original language	English
Pages (from-to)	e115
Journal	Nucleic Acids Research
Volume	31
Issue number	19
DOIs	https://doi.org/10.1093/nar/gng113
Publication status	Published - Oct 2003

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title = "A microarray-based high throughput molecular marker genotyping method: the tagged microarray marker (TAM) approach",

abstract = "A microarray-based method has been developed for scoring thousands of DNAs for a co-dominant molecular marker on a glass slide. The approach was developed to detect insertional polymorphism of transposons and works well with single nucleotide polymorphism (SNP) markers. Biotinterminated allele-specific PCR products are spotted unpurified onto streptavidin-coated glass slides and visualised by hybridisation of fluorescent detector oligonucleotides to tags attached to the allelespecific PCR primers. Two tagged primer oligonucleotides are used per locus and each tag is detected by hybridisation to a concatameric DNA probe labelled with multiple fluorochromes.",

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AU - Russell, Joanne

AU - Ellis, T. H. Noel

AU - Isaac, Peter

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AB - A microarray-based method has been developed for scoring thousands of DNAs for a co-dominant molecular marker on a glass slide. The approach was developed to detect insertional polymorphism of transposons and works well with single nucleotide polymorphism (SNP) markers. Biotinterminated allele-specific PCR products are spotted unpurified onto streptavidin-coated glass slides and visualised by hybridisation of fluorescent detector oligonucleotides to tags attached to the allelespecific PCR primers. Two tagged primer oligonucleotides are used per locus and each tag is detected by hybridisation to a concatameric DNA probe labelled with multiple fluorochromes.

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A microarray-based high throughput molecular marker genotyping method: the tagged microarray marker (TAM) approach

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