Objectives: To explore skin barrier and immune response biomarkers in stratumcorneum (SC) tape strips from children with AD with different skin types.
Methods: Tape strips were collected from lesional and nonlesional forearm skin of53 children with AD and 50 controls. We analysed 28 immunomodulatory mediators,and natural moisturizing factors (NMF) and corneocyte morphology.
Results: Interleukin (IL)-1b, IL-18, C-X-C motif chemokine (CXCL) 8 (CXCL8),C-C motif chemokine ligand (CCL) 22 (CCL22), CCL17, CXCL10 and CCL2 weresignificantly higher (P < 005) in lesional AD skin compared with nonlesionalAD skin; the opposite trend was seen for IL-1a. CXCL8, CCL2 and CCL17showed an association with objective SCORing Atopic Dermatitis score. NMFlevels showed a gradual decrease from healthy skin to nonlesional and lesionalAD skin. This gradual decreasing pattern was observed in skin type II but not inskin type VI. Skin type VI showed higher NMF levels in both nonlesional andlesional AD skin than skin type II. Corneocyte morphology was significantly differentin lesional AD skin compared with nonlesional AD and healthy skin.
Conclusions: Minimally invasive tape-stripping is suitable for the determination ofmany inflammatory mediators and skin barrier biomarkers in children with AD.This study shows differences between children with AD with skin type II andskin type VI in NMF levels, suggesting that some aspects of pathophysiologicalmechanisms may differ in AD children with light versus dark skin types.
- atopic dermatitis
- stratum corneum analysis
- natural moisturizing factor
- skin barrier
- skin type differences