A molecular explanation for the recessive nature of parkin-linked Parkinson's disease

Donald E. Spratt, R. Julio Martinez-Torres, Yeong J. Noh, Pascal Mercier, Noah Manczyk, Kathryn R. Barber, Jacob D. Aguirre, Lynn Burchell, Andrew Purkiss, Helen Walden (Lead / Corresponding author), Gary S. Shaw

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    Abstract

    Mutations in the park2 gene, encoding the RING-inBetweenRING-RING E3 ubiquitin ligase parkin, cause 50% of autosomal recessive juvenile Parkinsonism cases. More than 70 known pathogenic mutations occur throughout parkin, many of which cluster in the inhibitory amino-terminal ubiquitin-like domain, and the carboxy-terminal RING2 domain that is indispensable for ubiquitin transfer. A structural rationale showing how autosomal recessive juvenile Parkinsonism mutations alter parkin function is still lacking. Here we show that the structure of parkin RING2 is distinct from canonical RING E3 ligases and lacks key elements required for E2-conjugating enzyme recruitment. Several pathogenic mutations in RING2 alter the environment of a single surface-exposed catalytic cysteine to inhibit ubiquitination. Native parkin adopts a globular inhibited conformation in solution facilitated by the association of the ubiquitin-like domain with the RING-inBetweenRING-RING C-terminus. Autosomal recessive juvenile Parkinsonism mutations disrupt this conformation. Finally, parkin autoubiquitinates only in cis, providing a molecular explanation for the recessive nature of autosomal recessive juvenile Parkinsonism.

    Original languageEnglish
    Article number1983
    Number of pages12
    JournalNature Communications
    Volume4
    DOIs
    Publication statusPublished - 1 Jan 2013

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  • Cite this

    Spratt, D. E., Julio Martinez-Torres, R., Noh, Y. J., Mercier, P., Manczyk, N., Barber, K. R., Aguirre, J. D., Burchell, L., Purkiss, A., Walden, H., & Shaw, G. S. (2013). A molecular explanation for the recessive nature of parkin-linked Parkinson's disease. Nature Communications, 4, [1983]. https://doi.org/10.1038/ncomms2983