A Morphological identification cell cytotoxicity assay using cytoplasm-localized fluorescent probe (CLFP) to distinguish living and dead cells

Fangfang Lai, Zhengwei Shen, Hui Wen, Jialing Chen, Xiang Zhang, Ping Lin, Dali Yin, Huaqing Cui (Lead / Corresponding author), Xiaoguang Chen (Lead / Corresponding author)

Research output: Contribution to journalArticle

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Abstract

Cell cytotoxicity assays include cell activity assays and morphological identification assays. Currently, all frequently used cytotoxicity assays belong to cell activity assays but suffer from detection limitations. Morphological identification of cell death remains as the gold standard, although the method is difficult to scale up. At present there is no generally accepted morphological identification based cell cytotoxicity assay. In this study, we applied previous developed cell cytoplasm-localized fluorescent probe (CLFP) to display cell morphologies. Under fluorescence microscopy, the fluorescence morphology and intensity of living cells are distinct from dead cells. Based on these characters we extracted the images of living cells from series of samples via computational analysis. Thus, a novel cell morphological identification cytotoxicity assay (CLFP assay) is developed. The performance of the CLFP assay was similar to cell activity assay (MTT assay), but the accuracy of the CLFP assay was superior when measuring the cytotoxicity of active compounds.

Original languageEnglish
Pages (from-to)257-263
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume482
Issue number2
Early online date11 Nov 2016
DOIs
Publication statusPublished - 8 Jan 2017

Keywords

  • Cytotoxicity assay
  • Cell death
  • Cytoplasm-localized fluorescent probe
  • Cell morphological identification
  • Computational analysis

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