A multi-ethnic genome-wide association study identifies novel loci for non-syndromic cleft lip with or without cleft palate on 2p24.2, 17q23 and 19q13

Elizabeth J Leslie, Jenna C Carlson, John R Shaffer, Eleanor Feingold, George Wehby, Cecelia A Laurie, Deepti Jain, Cathy C Laurie, Kimberly F Doheny, Toby McHenry, Judith Resick, Carla Sanchez, Jennifer Jacobs, Beth Emanuele, Alexandre R Vieira, Katherine Neiswanger, Andrew C Lidral, Luz Consuelo Valencia-Ramirez, Ana Maria Lopez-Palacio, Dora Rivera ValenciaMauricio Arcos-Burgos, Andrew E Czeizel, L Leigh Field, Carmencita D Padilla, Eva Maria C Cutiongco-de la Paz, Frederic Deleyiannis, Kaare Christensen, Ronald G Munger, Rolv T Lie, Allen Wilcox, Paul A Romitti, Eduardo E Castilla, Juan C Mereb, Fernando A Poletta, Iêda M Orioli, Flavia M Carvalho, Jacqueline T Hecht, Susan H Blanton, Carmen J Buxó, Azeez Butali, Peter A Mossey, Wasiu L Adeyemo, Olutayo James, Ramat O Braimah, Babatunde S Aregbesola, Mekonen A Eshete, Fikre Abate, Mine Koruyucu, Figen Seymen, Lian Ma, Javier Enríquez de Salamanca, Seth M Weinberg, Lina Moreno, Jeffrey C Murray, Mary L Marazita

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78 Citations (Scopus)

Abstract

Orofacial clefts (OFCs), which include non-syndromic cleft lip with or without cleft palate (CL/P), are among the most common birth defects in humans, affecting approximately 1 in 700 newborns. CL/P is phenotypically heterogeneous and has a complex etiology caused by genetic and environmental factors. Previous genome-wide association studies (GWASs) have identified at least 15 risk loci for CL/P. As these loci do not account for all of the genetic variance of CL/P, we hypothesized the existence of additional risk loci. We conducted a multiethnic GWAS in 6480 participants (823 unrelated cases, 1700 unrelated controls and 1319 case-parent trios) with European, Asian, African and Central and South American ancestry. Our GWAS revealed novel associations on 2p24 near FAM49A, a gene of unknown function (P = 4.22 × 10(-8)), and 19q13 near RHPN2, a gene involved in organizing the actin cytoskeleton (P = 4.17 × 10(-8)). Other regions reaching genome-wide significance were 1p36 (PAX7), 1p22 (ARHGAP29), 1q32 (IRF6), 8q24 and 17p13 (NTN1), all reported in previous GWASs. Stratification by ancestry group revealed a novel association with a region on 17q23 (P = 2.92 × 10(-8)) among individuals with European ancestry. This region included several promising candidates including TANC2, an oncogene required for development, and DCAF7, a scaffolding protein required for craniofacial development. In the Central and South American ancestry group, significant associations with loci previously identified in Asian or European ancestry groups reflected their admixed ancestry. In summary, we have identified novel CL/P risk loci and suggest new genes involved in craniofacial development, confirming the highly heterogeneous etiology of OFCs.

Original languageEnglish
Pages (from-to)2862-2872
Number of pages11
JournalHuman Molecular Genetics
Volume25
Issue number13
Early online date30 Mar 2016
DOIs
Publication statusPublished - 1 Jul 2016

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    Leslie, E. J., Carlson, J. C., Shaffer, J. R., Feingold, E., Wehby, G., Laurie, C. A., Jain, D., Laurie, C. C., Doheny, K. F., McHenry, T., Resick, J., Sanchez, C., Jacobs, J., Emanuele, B., Vieira, A. R., Neiswanger, K., Lidral, A. C., Valencia-Ramirez, L. C., Lopez-Palacio, A. M., ... Marazita, M. L. (2016). A multi-ethnic genome-wide association study identifies novel loci for non-syndromic cleft lip with or without cleft palate on 2p24.2, 17q23 and 19q13. Human Molecular Genetics, 25(13), 2862-2872. https://doi.org/10.1093/hmg/ddw104