A multigene family encoding R-SNAREs in the ciliate Paramecium tetraurelia

Christina Schilde (Lead / Corresponding author), Thomas Wassmer, Joerg Mansfeld, Helmut Plattner, Roland Kissmehl

    Research output: Contribution to journalArticlepeer-review

    33 Citations (Scopus)

    Abstract

    SNARE proteins (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) mediate membrane interactions and are conventionally divided into Q-SNAREs and R-SNAREs according to the possession of a glutamine or arginine residue at the core of their SNARE domain. Here, we describe a set of R-SNAREs from the ciliate Paramecium tetraurelia consisting of seven families encoded by 12 genes that are expressed simultaneously. The complexity of the endomembrane system in Paramecium can explain this high number of genes. All P. tetraurelia synaptobrevins (PtSybs) possess a SNARE domain and show homology to the Longin family of R-SNAREs such as Ykt6, Sec22 and tetanus toxin-insensitive VAMP (TI-VAMP). We localized four exemplary PtSyb subfamilies with GFP constructs and antibodies on the light and electron microscopic level. PtSyb1-1, PtSyb1-2 and PtSyb3-1 were found in the endoplasmic reticulum, whereas PtSyb2 is localized exclusively in the contractile vacuole complex. PtSyb6 was found cytosolic but also resides in regularly arranged structures at the cell cortex (parasomal sacs), the cytoproct and oral apparatus, probably representing endocytotic compartments. With gene silencing, we showed that the R-SNARE of the contractile vacuole complex, PtSyb2, functions to maintain structural integrity as well as functionality of the osmoregulatory system but also affects cell division.

    Original languageEnglish
    Pages (from-to)440-455
    Number of pages16
    JournalTraffic
    Volume7
    Issue number4
    Early online date14 Mar 2006
    DOIs
    Publication statusPublished - 14 Mar 2006

    Keywords

    • Ciliates
    • Membrane trafficking
    • Paramecium
    • R-SNAREs
    • Regulated secretion
    • SNAREs
    • v-SNAREs

    ASJC Scopus subject areas

    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology

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