A new proteinaceous pathogen-associated molecular pattern (PAMP) identified in Ascomycete fungi induces cell death in Solanaceae

Barbara Franco-Orozco, Adokiye Berepiki, Olaya Ruiz, Louise Gamble, Lucie L. Griffe, Shumei Wang, Paul R. J. Birch, Kostya Kanyuka, Anna Avrova (Lead / Corresponding author)

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Pathogen-associated molecular patterns (PAMPs) are detected by plant pattern recognition receptors (PRRs), which gives rise to PAMP-triggered immunity (PTI). We characterized a novel fungal PAMP, Cell Death Inducing 1 (RcCDI1), identified in the Rhynchosporium commune transcriptome sampled at an early stage of barley (Hordeum vulgare) infection. The ability of RcCDI1 and its homologues from different fungal species to induce cell death in Nicotiana benthamiana was tested following agroinfiltration or infiltration of recombinant proteins produced by Pichia pastoris. Virus-induced gene silencing (VIGS) and transient expression of Phytophthora infestans effectors PiAVR3a and PexRD2 were used to assess the involvement of known components of PTI in N. benthamiana responses to RcCDI1. RcCDI1 was highly upregulated early during barley colonization with R. commune. RcCDI1 and its homologues from different fungal species, including Zymoseptoria tritici, Magnaporthe oryzae and Neurospora crassa, exhibited PAMP activity, inducing cell death in Solanaceae but not in other families of dicots or monocots. RcCDI1-triggered cell death was shown to require N. benthamiana Brassinosteroid insensitive 1-Associated Kinase 1 (NbBAK1), N. benthamiana suppressor of BIR1-1 (NbSOBIR1) and N. benthamiana SGT1 (NbSGT1), but was not suppressed by PiAVR3a or PexRD2. We report the identification of a novel Ascomycete PAMP, RcCDI1, recognized by Solanaceae but not by monocots, which activates cell death through a pathway that is distinct from that triggered by the oomycete PAMP INF1.

Original languageEnglish
Pages (from-to)1657-1672
Number of pages16
JournalNew Phytologist
Volume214
Issue number4
Early online date7 Apr 2017
DOIs
Publication statusPublished - Jun 2017

Keywords

  • Journal article
  • Ascomycete
  • Cell death
  • Elicitor
  • Fungal
  • Pathogen
  • Pathogen-associated molecular pattern (PAMP)
  • Rhynchosporium commune
  • Solanaceae

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    Student Theses

    Secretion and delivery of virulence proteins from Phytophthora infestans to its host

    Author: Wang, S., 2019

    Supervisor: Birch, P. (Supervisor), Boevink, P. (Supervisor) & Whisson, S. (Supervisor)

    Student thesis: Doctoral ThesisDoctor of Philosophy

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    Birch, Paul

    Person: Academic

    Cite this

    Franco-Orozco, B., Berepiki, A., Ruiz, O., Gamble, L., Griffe, L. L., Wang, S., Birch, P. R. J., Kanyuka, K., & Avrova, A. (2017). A new proteinaceous pathogen-associated molecular pattern (PAMP) identified in Ascomycete fungi induces cell death in Solanaceae. New Phytologist, 214(4), 1657-1672. https://doi.org/10.1111/nph.14542