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Microbe-based decontamination of phenol-polluted environments has significant advantages over physical and chemical approaches by being relatively cheaper and ensuring complete phenol degradation. There is a need to search for commercially prospective bacterial strains that are resistant to phenol and other co-pollutants, e.g. oil hydrocarbons, in contaminated environments, and able to carry out efficient phenol biodegradation at a variable range of concentrations. This research characterizes the phenol-biodegrading ability of a new actinobacteria strain isolated from a lubricant-contaminated soil environment. Phenotypic and phylogenetic analyses showed that the novel strain UCM Ac-603 belonged to the species Rhodococcus aetherivorans, and phenol degrading ability was quantitatively characterized for the first time. R. aetherivorans UCM Ac-603 tolerated and assimilated phenol (100% of supplied concentration) and various hydrocarbons (56.2–94.4%) as sole carbon sources. Additional nutrient supplementation was not required for degradation and this organism could grow at a phenol concentration of 500 mg L −1 without inhibition. Complete phenol assimilation occurred after 4 days at an initial concentration of 1750 mg L −1 for freely-suspended cells and at 2000 mg L −1 for vermiculite-immobilized cells: 99.9% assimilation of phenol was possible from a total concentration of 3000 mg L −1 supplied at daily fractional phenol additions of 750 mg L −1 over 4 days. In terms of phenol degradation rates, R. aetherivorans UCM Ac-602 showed efficient phenol degradation over a wide range of initial concentrations with the rates (e.g. 35.7 mg L −1 h −1 at 500 mg L −1 phenol, and 18.2 mg L −1 h −1 at 1750 mg L −1 phenol) significantly exceeding (1.2–5 times) reported data for almost all other phenol-assimilating bacteria. Such efficient phenol degradation ability compared to currently known strains and other beneficial characteristics of R. aetherivorans UCM Ac-602 suggest it is a promising candidate for bioremediation of phenol-contaminated environments.
|Number of pages||15|
|Journal||Applied Microbiology and Biotechnology|
|Early online date||11 Feb 2020|
|Publication status||Published - 1 Apr 2020|
- Cell immobilization
- Phenol biodegradation
- Rhodococcus aetherivorans
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
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- 2 Finished
COG3: The Geology, Geometallurgy and Geomicrobiology of Cobalt Resources Leading to New Product Streams (joint with Natural History Museum and Universities of Manchester, Bangor, Exeter, Loughborough and Southampton and Industrial Partner)
Natural Environment Research Council
1/05/15 → 31/03/21
- 17 Citations
- 1 Article
Correction to: a new Rhodococcus aetherivorans strain isolated from lubricant-contaminated soil as a prospective phenol-biodegrading agentNogina, T., Fomina, M., Dumanskaya, T., Zelena, L., Khomenko, L., Mikhalovsky, S., Podgorskyi, V. & Gadd, G. M., 1 Apr 2020, In: Applied Microbiology and Biotechnology. 104, 8, p. 3689-3690 2 p.
Research output: Contribution to journal › Article › peer-reviewOpen Access