A normalised scale for structural genomics target ranking: the OB-Score

Ian M. Overton; Geoffrey J. Barton

doi:10.1016/j.febslet.2006.06.015

A normalised scale for structural genomics target ranking: the OB-Score

Ian M. Overton, Geoffrey J. Barton (Lead / Corresponding author)

Computational Biology

Research output: Contribution to journal › Article › peer-review

56 Citations (Scopus)

Abstract

Target selection and ranking is fundamental to structural genomics. We present a Z-score scale, the "OB-Score", to rank potential targets by their predicted propensity to produce diffraction-quality crystals. The OB-Score is derived from a matrix of predicted isoelectric point and hydrophobicity values for nonredundant PDB entries solved to <or=3.0 A against a background of UniRef50. A highly significant difference was found between the OB-Scores for TargetDB test datasets. A wide range of OB-Scores was observed across 241 proteomes and within 7868 PfamA families; 73.4% of PfamA families contain >or=1 member with a high OB-Score, presenting favourable candidates for structural studies.

Original language	English
Pages (from-to)	4005-4009
Number of pages	5
Journal	FEBS Letters
Volume	580
Issue number	16
Early online date	16 Jun 2006
DOIs	https://doi.org/10.1016/j.febslet.2006.06.015
Publication status	Published - 10 Jul 2006

Keywords

Databases, Protein
Genomics
Hydrophobic and hydrophilic Interactions
Isoelectric point
Proteins
Statistics as topic

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10.1016/j.febslet.2006.06.015

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AB - Target selection and ranking is fundamental to structural genomics. We present a Z-score scale, the "OB-Score", to rank potential targets by their predicted propensity to produce diffraction-quality crystals. The OB-Score is derived from a matrix of predicted isoelectric point and hydrophobicity values for nonredundant PDB entries solved to or=1 member with a high OB-Score, presenting favourable candidates for structural studies.

KW - Databases, Protein

KW - Genomics

KW - Hydrophobic and hydrophilic Interactions

KW - Isoelectric point

KW - Proteins

KW - Statistics as topic

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DO - 10.1016/j.febslet.2006.06.015

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JO - FEBS Letters

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A normalised scale for structural genomics target ranking: the OB-Score

Abstract

Keywords

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