A normalised scale for structural genomics target ranking: the OB-Score

Ian M. Overton; Geoffrey J. Barton

doi:10.1016/j.febslet.2006.06.015

A normalised scale for structural genomics target ranking: the OB-Score

Ian M. Overton
, Geoffrey J. Barton (Lead / Corresponding author)

Computational Biology

Research output: Contribution to journal › Article › peer-review

57 Citations (Scopus)

Abstract

Target selection and ranking is fundamental to structural genomics. We present a Z-score scale, the "OB-Score", to rank potential targets by their predicted propensity to produce diffraction-quality crystals. The OB-Score is derived from a matrix of predicted isoelectric point and hydrophobicity values for nonredundant PDB entries solved to <or=3.0 A against a background of UniRef50. A highly significant difference was found between the OB-Scores for TargetDB test datasets. A wide range of OB-Scores was observed across 241 proteomes and within 7868 PfamA families; 73.4% of PfamA families contain >or=1 member with a high OB-Score, presenting favourable candidates for structural studies.

Original language	English
Pages (from-to)	4005-4009
Number of pages	5
Journal	FEBS Letters
Volume	580
Issue number	16
Early online date	16 Jun 2006
DOIs	https://doi.org/10.1016/j.febslet.2006.06.015
Publication status	Published - 10 Jul 2006

Keywords

Databases, Protein
Genomics
Hydrophobic and hydrophilic Interactions
Isoelectric point
Proteins
Statistics as topic

Access to Document

10.1016/j.febslet.2006.06.015

Cite this

@article{56b53869e4d54dcc9b7b821b030b8a67,

title = "A normalised scale for structural genomics target ranking: the OB-Score",

abstract = "Target selection and ranking is fundamental to structural genomics. We present a Z-score scale, the {"}OB-Score{"}, to rank potential targets by their predicted propensity to produce diffraction-quality crystals. The OB-Score is derived from a matrix of predicted isoelectric point and hydrophobicity values for nonredundant PDB entries solved to or=1 member with a high OB-Score, presenting favourable candidates for structural studies.",

keywords = "Databases, Protein, Genomics, Hydrophobic and hydrophilic Interactions, Isoelectric point, Proteins, Statistics as topic",

author = "Overton, \{Ian M.\} and Barton, \{Geoffrey J.\}",

year = "2006",

month = jul,

day = "10",

doi = "10.1016/j.febslet.2006.06.015",

language = "English",

volume = "580",

pages = "4005--4009",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley",

number = "16",

}

TY - JOUR

T1 - A normalised scale for structural genomics target ranking

T2 - the OB-Score

AU - Overton, Ian M.

AU - Barton, Geoffrey J.

PY - 2006/7/10

Y1 - 2006/7/10

N2 - Target selection and ranking is fundamental to structural genomics. We present a Z-score scale, the "OB-Score", to rank potential targets by their predicted propensity to produce diffraction-quality crystals. The OB-Score is derived from a matrix of predicted isoelectric point and hydrophobicity values for nonredundant PDB entries solved to or=1 member with a high OB-Score, presenting favourable candidates for structural studies.

AB - Target selection and ranking is fundamental to structural genomics. We present a Z-score scale, the "OB-Score", to rank potential targets by their predicted propensity to produce diffraction-quality crystals. The OB-Score is derived from a matrix of predicted isoelectric point and hydrophobicity values for nonredundant PDB entries solved to or=1 member with a high OB-Score, presenting favourable candidates for structural studies.

KW - Databases, Protein

KW - Genomics

KW - Hydrophobic and hydrophilic Interactions

KW - Isoelectric point

KW - Proteins

KW - Statistics as topic

U2 - 10.1016/j.febslet.2006.06.015

DO - 10.1016/j.febslet.2006.06.015

M3 - Article

C2 - 16808918

SN - 0014-5793

VL - 580

SP - 4005

EP - 4009

JO - FEBS Letters

JF - FEBS Letters

IS - 16

ER -

A normalised scale for structural genomics target ranking: the OB-Score

Abstract

Keywords

Access to Document

Fingerprint

Cite this