A novel and fast purification method for nucleoside transporters

Zhenyu Hao, Maren Thomsen, Vincent L. G. Postis, Amelia Lesiuk, David Sharples, Yingying Wang, Mark Bartlam (Lead / Corresponding author), Adrian Goldman (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Nucleoside transporters (NTs) play critical biological roles in humans, and to understand the molecular mechanism of nucleoside transport requires high-resolution structural information. However, the main bottleneck for structural analysis of NTs is the production of pure, stable, and high quality native protein for crystallization trials. Here we report a novel membrane protein expression and purification strategy, including construction of a high-yield membrane protein expression vector, and a new and fast purification protocol for NTs. The advantages of this strategy are the improved time efficiency, leading to high quality, active, stable membrane proteins, and the efficient use of reagents and consumables. Our strategy might serve as a useful point of reference for investigating NTs and other membrane proteins by clarifying the technical points of vector construction and improvements of membrane protein expression and purification.
Original languageEnglish
Article number23
Number of pages8
JournalFront. Mol. Biosci.
Publication statusPublished - 9 Jun 2016


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