A novel method to quantify dentine tubule occlusion applied to in situ model samples

R C Olley, Charles R. Parkinson, R Wilson, Rebecca Moazzez, D Bartlett

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


OBJECTIVES: To develop an innovative computerised routine, in conjunction with a minimally destructive imaging technique, to quantify dentine tubule occlusion.

METHODS: Polished human dentine samples (n = 480) were brushed during a 4-day in situ study with two occlusion-based dentifrices, a sodium fluoride control dentifrice (1,450 ppm) or water. Samples were imaged with tandem scanning microscopy (TSM) and conventional scanning electron microscopy (SEM). The level of dentine patency was then assessed using a visual ordinal scale ('standard') or quantitatively using a specially designed computational routine.

RESULTS: The occlusion-based dentifrice resulted in significantly less patent dentine than controls for the 'standard' (p = 0.01) assessment, but not for computer analyses (p = 0.10). The correlation of the number of individual patent tubules counted visually and by the computational routine in calibrations and in the in situ study was ≥ 0.8.

CONCLUSIONS: This study identified a new computer-based routine, capable of objectively quantifying the patency of dentine imaged by SEM and TSM.

Original languageEnglish
Pages (from-to)69-72
Number of pages4
JournalCaries Research
Issue number1
Publication statusPublished - Jan 2014


  • Algorithms
  • Cariostatic Agents
  • Dentifrices
  • Dentin
  • Dentin Desensitizing Agents
  • Glass
  • Humans
  • Image Processing, Computer-Assisted
  • Microscopy
  • Microscopy, Electron, Scanning
  • Observer Variation
  • Pattern Recognition, Automated
  • Pattern Recognition, Visual
  • Reproducibility of Results
  • Sodium Fluoride
  • Software
  • Water
  • Journal Article
  • Research Support, Non-U.S. Gov't


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