TY - JOUR
T1 - A novel role for dpp in the shaping of bivalve shells revealed in a conserved molluscan developmental program
AU - Kin, Koryu
AU - Kakoi, Shota
AU - Wada, Hiroshi
PY - 2009/5/1
Y1 - 2009/5/1
N2 - During the molluscan evolution leading to the bivalves, the single dorsal shell was doubled. To elucidate the molecular developmental basis underlying this prominent morphological transition, we described the cell cleavage and expression patterns of three genes, brachyury, engrailed, and dpp in the Japanese spiny oyster Saccostrea kegaki, and examined the function of dpp in this species. The cleavage pattern of the S. kegaki embryo was nearly the same as the previously described pattern of other bivalve species, suggesting that the pattern itself is highly important for the establishment or the maintenance of the bivalve body plan. The expression pattern of a brachyury homolog in S. kegaki (SkBra) was similar to the pattern in gastopods even at the single cell level despite the deep divergence of gastropods and bivalves. Engrailed and dpp were previously found to be expressed around the shell anlagen in gastropods. Like that of gastropods, an engrailed homolog in S. kegaki (SkEn) was found to be expressed around the shell anlagen. However, the dpp homolog in S. kegaki (SkDpp) was expressed only in the cells along the dorsal midline. ZfBMP4 treatment experiments revealed the importance of dpp in establishing the characteristic shape of the bivalve shell anlagen.
AB - During the molluscan evolution leading to the bivalves, the single dorsal shell was doubled. To elucidate the molecular developmental basis underlying this prominent morphological transition, we described the cell cleavage and expression patterns of three genes, brachyury, engrailed, and dpp in the Japanese spiny oyster Saccostrea kegaki, and examined the function of dpp in this species. The cleavage pattern of the S. kegaki embryo was nearly the same as the previously described pattern of other bivalve species, suggesting that the pattern itself is highly important for the establishment or the maintenance of the bivalve body plan. The expression pattern of a brachyury homolog in S. kegaki (SkBra) was similar to the pattern in gastopods even at the single cell level despite the deep divergence of gastropods and bivalves. Engrailed and dpp were previously found to be expressed around the shell anlagen in gastropods. Like that of gastropods, an engrailed homolog in S. kegaki (SkEn) was found to be expressed around the shell anlagen. However, the dpp homolog in S. kegaki (SkDpp) was expressed only in the cells along the dorsal midline. ZfBMP4 treatment experiments revealed the importance of dpp in establishing the characteristic shape of the bivalve shell anlagen.
KW - Bivalve
KW - Brachyury
KW - Cell cleavage pattern
KW - Dpp
KW - Engrailed
KW - Mollusc
KW - Saccostrea kegaki
KW - Shell
UR - http://www.scopus.com/inward/record.url?scp=64049084625&partnerID=8YFLogxK
U2 - 10.1016/j.ydbio.2009.01.021
DO - 10.1016/j.ydbio.2009.01.021
M3 - Article
C2 - 19382296
AN - SCOPUS:64049084625
VL - 329
SP - 152
EP - 166
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 1
ER -