A novel TBP-associated factor of SL1 functions in RNA polymerase I transcription

Julia J. Gorski, Shalini Pathak, Kostya Panov, Taciana Kasciukovic, Tanya Panova, Jacqueline Russell, Joost C. B. M. Zomerdijk

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    72 Citations (Scopus)


    In mammalian RNA polymerase I transcription, SL1, an assembly of TBP and associated factors (TAFs), is essential for preinitiation complex formation at ribosomal RNA gene promoters in vitro. We provide evidence for a novel component of SL1, TAFI41 (MGC5306), which functions in Pol I transcription. TAFI41 resides at the rDNA promoter in the nucleolus and co-purifies and co-immunoprecipitates with SL1. TAFI41 immunodepletion from nuclear extracts dramatically reduces Pol I transcription; addition of SL1 restores the ability of these extracts to support Pol I transcription. In cells, siRNA-mediated decreased expression of TAFI41 leads to loss of SL1 from the rDNA promoter in vivo, with concomitant loss of Pol I from the rDNA and reduced synthesis of the pre-rRNA. Extracts from these cells support reduced levels of Pol I transcription; addition of SL1 to the extracts raises the level of Pol I transcription. These data suggest that TAFI41 is integral to transcriptionally active SL1 and imply a role for SL1, including the TAFI41 subunit, in Pol I recruitment and, therefore, preinitiation complex formation in vivo.
    Original languageEnglish
    Pages (from-to)1560-1568
    Number of pages9
    JournalThe EMBO Journal
    Issue number6
    Publication statusPublished - Mar 2007


    • Ribosomal RNA synthesis
    • RNA polymerase I
    • RNA polymerase recruitment
    • SL1
    • TAFI41


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