A number of subgenomic DNAs are produced following agroinoculation of plants with beet curly top virus

Drake C. Stenger, Matthew C. Stevenson , Sheriar G. Hormuzdi, David M. Bisaro

    Research output: Contribution to journalArticle

    50 Citations (Scopus)

    Abstract

    In addition to ss and ds genomic DNA, agroinoculation of Nicotiana benthamiana plants with the Logan strain of the geminivirus beet curly top virus (BCTV) consistently resulted in de novo production of subgenomic DNAs on initial passage. Single-stranded and dsDNA forms representing at least seven size classes (0-8 to 1-8 kb) of subgenomic DNA were observed in total DNA extracts from inoculated plants. Extracts from infected sugar beet and tomato contained variable but usually smaller amounts of subgenomic DNAs, suggesting that their production may be influenced by the host species. Restriction endonuclease mapping and partial nucleotide sequencing of three independent clones of a 1- 5 kb size class indicated that this subgenomic DNA is produced from the standard viral genome by two separate deletion events. One deletion of 941 bp includes portions of the leftward open reading frames (ORFs) L1, L2 and L3, while the other deletion of 579 bp encompasses portions of the intergenic region and the rightward ORFs R1, R2 and R3. The data indicate that the 1-5 kb BCTV subgenomic DNA is a defective DNA that has retained ciselements essential for replication.

    Original languageEnglish
    Pages (from-to)237-242
    Number of pages6
    JournalJournal of General Virology
    Volume73
    Issue number2
    DOIs
    Publication statusPublished - 1 Feb 1992

    Fingerprint

    Beta vulgaris
    Viruses
    DNA
    Open Reading Frames
    Geminiviridae
    Restriction Mapping
    Intergenic DNA
    DNA Viruses
    Viral Genome
    Plant Extracts
    Lycopersicon esculentum
    Tobacco
    Nucleotides
    Clone Cells

    Cite this

    Stenger, Drake C. ; Stevenson , Matthew C. ; Hormuzdi, Sheriar G. ; Bisaro, David M. / A number of subgenomic DNAs are produced following agroinoculation of plants with beet curly top virus. In: Journal of General Virology. 1992 ; Vol. 73, No. 2. pp. 237-242.
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    abstract = "In addition to ss and ds genomic DNA, agroinoculation of Nicotiana benthamiana plants with the Logan strain of the geminivirus beet curly top virus (BCTV) consistently resulted in de novo production of subgenomic DNAs on initial passage. Single-stranded and dsDNA forms representing at least seven size classes (0-8 to 1-8 kb) of subgenomic DNA were observed in total DNA extracts from inoculated plants. Extracts from infected sugar beet and tomato contained variable but usually smaller amounts of subgenomic DNAs, suggesting that their production may be influenced by the host species. Restriction endonuclease mapping and partial nucleotide sequencing of three independent clones of a 1- 5 kb size class indicated that this subgenomic DNA is produced from the standard viral genome by two separate deletion events. One deletion of 941 bp includes portions of the leftward open reading frames (ORFs) L1, L2 and L3, while the other deletion of 579 bp encompasses portions of the intergenic region and the rightward ORFs R1, R2 and R3. The data indicate that the 1-5 kb BCTV subgenomic DNA is a defective DNA that has retained ciselements essential for replication.",
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    A number of subgenomic DNAs are produced following agroinoculation of plants with beet curly top virus. / Stenger, Drake C.; Stevenson , Matthew C. ; Hormuzdi, Sheriar G.; Bisaro, David M.

    In: Journal of General Virology, Vol. 73, No. 2, 01.02.1992, p. 237-242.

    Research output: Contribution to journalArticle

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    AU - Bisaro, David M.

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    N2 - In addition to ss and ds genomic DNA, agroinoculation of Nicotiana benthamiana plants with the Logan strain of the geminivirus beet curly top virus (BCTV) consistently resulted in de novo production of subgenomic DNAs on initial passage. Single-stranded and dsDNA forms representing at least seven size classes (0-8 to 1-8 kb) of subgenomic DNA were observed in total DNA extracts from inoculated plants. Extracts from infected sugar beet and tomato contained variable but usually smaller amounts of subgenomic DNAs, suggesting that their production may be influenced by the host species. Restriction endonuclease mapping and partial nucleotide sequencing of three independent clones of a 1- 5 kb size class indicated that this subgenomic DNA is produced from the standard viral genome by two separate deletion events. One deletion of 941 bp includes portions of the leftward open reading frames (ORFs) L1, L2 and L3, while the other deletion of 579 bp encompasses portions of the intergenic region and the rightward ORFs R1, R2 and R3. The data indicate that the 1-5 kb BCTV subgenomic DNA is a defective DNA that has retained ciselements essential for replication.

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