A physical, genetic and functional sequence assembly of the barley genome

Klaus F. X. Mayer (Lead / Corresponding author), Robbie Waugh (Lead / Corresponding author), John W. S. Brown, Alan Schulman, Peter Langridge, Matthias Platzer, Geoffrey B. Fincher, Gary J. Muehlbauer, Kazuhiro Sato, Timothy J. Close, Roger P. Wise, Nils Stein (Lead / Corresponding author), International Barley Genome Sequencing Consortium

    Research output: Contribution to journalArticlepeer-review

    976 Citations (Scopus)

    Abstract

    Barley (Hordeum vulgare L.) is among the world's earliest domesticated and most important crop plants. It is diploid with a large haploid genome of 5.1 gigabases (Gb). Here we present an integrated and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a structured whole-genome context. We developed a physical map of 4.98?Gb, with more than 3.90?Gb anchored to a high-resolution genetic map. Projecting a deep whole-genome shotgun assembly, complementary DNA and deep RNA sequence data onto this framework supports 79,379 transcript clusters, including 26,159 'high-confidence' genes with homology support from other plant genomes. Abundant alternative splicing, premature termination codons and novel transcriptionally active regions suggest that post-transcriptional processing forms an important regulatory layer. Survey sequences from diverse accessions reveal a landscape of extensive single-nucleotide variation. Our data provide a platform for both genome-assisted research and enabling contemporary crop improvement.
    Original languageEnglish
    Pages (from-to)711-716
    Number of pages6
    JournalNature
    Volume491
    Issue number7426
    DOIs
    Publication statusPublished - 29 Nov 2012

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