A physical, genetic and functional sequence assembly of the barley genome

Klaus F. X. Mayer (Lead / Corresponding author), Robbie Waugh (Lead / Corresponding author), John W. S. Brown, Alan Schulman, Peter Langridge, Matthias Platzer, Geoffrey B. Fincher, Gary J. Muehlbauer, Kazuhiro Sato, Timothy J. Close, Roger P. Wise, Nils Stein (Lead / Corresponding author), International Barley Genome Sequencing Consortium

    Research output: Contribution to journalArticle

    948 Citations (Scopus)

    Abstract

    Barley (Hordeum vulgare L.) is among the world's earliest domesticated and most important crop plants. It is diploid with a large haploid genome of 5.1 gigabases (Gb). Here we present an integrated and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a structured whole-genome context. We developed a physical map of 4.98?Gb, with more than 3.90?Gb anchored to a high-resolution genetic map. Projecting a deep whole-genome shotgun assembly, complementary DNA and deep RNA sequence data onto this framework supports 79,379 transcript clusters, including 26,159 'high-confidence' genes with homology support from other plant genomes. Abundant alternative splicing, premature termination codons and novel transcriptionally active regions suggest that post-transcriptional processing forms an important regulatory layer. Survey sequences from diverse accessions reveal a landscape of extensive single-nucleotide variation. Our data provide a platform for both genome-assisted research and enabling contemporary crop improvement.
    Original languageEnglish
    Pages (from-to)711-716
    Number of pages6
    JournalNature
    Volume491
    Issue number7426
    DOIs
    Publication statusPublished - 29 Nov 2012

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  • Cite this

    Mayer, K. F. X., Waugh, R., Brown, J. W. S., Schulman, A., Langridge, P., Platzer, M., Fincher, G. B., Muehlbauer, G. J., Sato, K., Close, T. J., Wise, R. P., Stein, N., & International Barley Genome Sequencing Consortium (2012). A physical, genetic and functional sequence assembly of the barley genome. Nature, 491(7426), 711-716. https://doi.org/10.1038/nature11543