A phytohaemagglutinin from the Azolla-anabaena symbiosis

R. B. Mellor; G. M. Gadd; P. Rowell; W. D.P. Stewart

doi:10.1016/0006-291X(81)90767-1

A phytohaemagglutinin from the Azolla-anabaena symbiosis

R. B. Mellor, G. M. Gadd, P. Rowell, W. D.P. Stewart

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Abstract

Proteins recovered from cell-free extracts of the Azolla-Anabaena azollae symbiosis exhibited haemagglutination activity; galactose was the most effective carbohydrate tested in preventing haemagglutination. Extracts of cyanobacteria-free Azolla also caused haemagglutination but extracts of free-living or symbiotic Anabaena azollae did not. Symbiotic Azolla plants grown on NO₃^- showed lower haemagglutination activity than did those grown on N₂; activity increased on removal of NO₃^-. The lower activity of the NO₃^--grown material may be due to NO₃su- exerting a direct effect on lectin activity/synthesis, or it may act indirectly by inhibiting the development of Anabaena which in turn affects the Azolla lectin. The purified lectin was shown to be composed of 6 sub-units, each of M.W. 21000.

Original language	English
Pages (from-to)	1348-1353
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	99
Issue number	4
DOIs	https://doi.org/10.1016/0006-291X(81)90767-1
Publication status	Published - 30 Apr 1981

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "A phytohaemagglutinin from the Azolla-anabaena symbiosis",

abstract = "Proteins recovered from cell-free extracts of the Azolla-Anabaena azollae symbiosis exhibited haemagglutination activity; galactose was the most effective carbohydrate tested in preventing haemagglutination. Extracts of cyanobacteria-free Azolla also caused haemagglutination but extracts of free-living or symbiotic Anabaena azollae did not. Symbiotic Azolla plants grown on NO3- showed lower haemagglutination activity than did those grown on N2; activity increased on removal of NO3-. The lower activity of the NO3--grown material may be due to NO3su- exerting a direct effect on lectin activity/synthesis, or it may act indirectly by inhibiting the development of Anabaena which in turn affects the Azolla lectin. The purified lectin was shown to be composed of 6 sub-units, each of M.W. 21000.",

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N2 - Proteins recovered from cell-free extracts of the Azolla-Anabaena azollae symbiosis exhibited haemagglutination activity; galactose was the most effective carbohydrate tested in preventing haemagglutination. Extracts of cyanobacteria-free Azolla also caused haemagglutination but extracts of free-living or symbiotic Anabaena azollae did not. Symbiotic Azolla plants grown on NO3- showed lower haemagglutination activity than did those grown on N2; activity increased on removal of NO3-. The lower activity of the NO3--grown material may be due to NO3su- exerting a direct effect on lectin activity/synthesis, or it may act indirectly by inhibiting the development of Anabaena which in turn affects the Azolla lectin. The purified lectin was shown to be composed of 6 sub-units, each of M.W. 21000.

AB - Proteins recovered from cell-free extracts of the Azolla-Anabaena azollae symbiosis exhibited haemagglutination activity; galactose was the most effective carbohydrate tested in preventing haemagglutination. Extracts of cyanobacteria-free Azolla also caused haemagglutination but extracts of free-living or symbiotic Anabaena azollae did not. Symbiotic Azolla plants grown on NO3- showed lower haemagglutination activity than did those grown on N2; activity increased on removal of NO3-. The lower activity of the NO3--grown material may be due to NO3su- exerting a direct effect on lectin activity/synthesis, or it may act indirectly by inhibiting the development of Anabaena which in turn affects the Azolla lectin. The purified lectin was shown to be composed of 6 sub-units, each of M.W. 21000.

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A phytohaemagglutinin from the Azolla-anabaena symbiosis

Abstract

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