A planar Airy beam light-sheet for two-photon microscopy

Neveen A. Hosny, James A. Seyforth, Gunnar Spickermann, Thomas J. Mitchell, Pedro Almada, Robert Chesters, Scott J. Mitchell, George Chennell, Anthony C. Vernon, Kwangwook Cho, Deepak P. Srivastava, Robert Forster, Tom Vettenburg (Lead / Corresponding author)

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Abstract

We demonstrate the first planar Airy light-sheet microscope. Fluorescence light-sheet microscopy has become the method of choice to study large biological samples with cellular or sub-cellular resolution. The propagation-invariant Airy beam enables a ten-fold increase in field-of-view with single-photon excitation; however, the characteristic asymmetry of the light-sheet limits its potential for multi-photon excitation. Here we show how a planar light-sheet can be formed from the curved propagation-invariant Airy beam. The resulting symmetric light sheet excites two-photon fluorescence uniformly across an extended field-of-view without the need for deconvolution. We demonstrate the method for rapid two-photon imaging of large volumes of neuronal tissue.
Original languageEnglish
Number of pages7
JournalarXiv
Publication statusPublished - 19 Jan 2020

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light beams
microscopy
photons
field of view
fluorescence
propagation
excitation
microscopes
asymmetry

Keywords

  • physics
  • optics

Cite this

Hosny, N. A., Seyforth, J. A., Spickermann, G., Mitchell, T. J., Almada, P., Chesters, R., ... Vettenburg, T. (2020). A planar Airy beam light-sheet for two-photon microscopy. arXiv.
Hosny, Neveen A. ; Seyforth, James A. ; Spickermann, Gunnar ; Mitchell, Thomas J. ; Almada, Pedro ; Chesters, Robert ; Mitchell, Scott J. ; Chennell, George ; Vernon, Anthony C. ; Cho, Kwangwook ; Srivastava, Deepak P. ; Forster, Robert ; Vettenburg, Tom. / A planar Airy beam light-sheet for two-photon microscopy. In: arXiv. 2020.
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abstract = "We demonstrate the first planar Airy light-sheet microscope. Fluorescence light-sheet microscopy has become the method of choice to study large biological samples with cellular or sub-cellular resolution. The propagation-invariant Airy beam enables a ten-fold increase in field-of-view with single-photon excitation; however, the characteristic asymmetry of the light-sheet limits its potential for multi-photon excitation. Here we show how a planar light-sheet can be formed from the curved propagation-invariant Airy beam. The resulting symmetric light sheet excites two-photon fluorescence uniformly across an extended field-of-view without the need for deconvolution. We demonstrate the method for rapid two-photon imaging of large volumes of neuronal tissue.",
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author = "Hosny, {Neveen A.} and Seyforth, {James A.} and Gunnar Spickermann and Mitchell, {Thomas J.} and Pedro Almada and Robert Chesters and Mitchell, {Scott J.} and George Chennell and Vernon, {Anthony C.} and Kwangwook Cho and Srivastava, {Deepak P.} and Robert Forster and Tom Vettenburg",
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Hosny, NA, Seyforth, JA, Spickermann, G, Mitchell, TJ, Almada, P, Chesters, R, Mitchell, SJ, Chennell, G, Vernon, AC, Cho, K, Srivastava, DP, Forster, R & Vettenburg, T 2020, 'A planar Airy beam light-sheet for two-photon microscopy', arXiv.

A planar Airy beam light-sheet for two-photon microscopy. / Hosny, Neveen A.; Seyforth, James A.; Spickermann, Gunnar; Mitchell, Thomas J.; Almada, Pedro; Chesters, Robert; Mitchell, Scott J.; Chennell, George; Vernon, Anthony C.; Cho, Kwangwook; Srivastava, Deepak P.; Forster, Robert; Vettenburg, Tom (Lead / Corresponding author).

In: arXiv, 19.01.2020.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A planar Airy beam light-sheet for two-photon microscopy

AU - Hosny, Neveen A.

AU - Seyforth, James A.

AU - Spickermann, Gunnar

AU - Mitchell, Thomas J.

AU - Almada, Pedro

AU - Chesters, Robert

AU - Mitchell, Scott J.

AU - Chennell, George

AU - Vernon, Anthony C.

AU - Cho, Kwangwook

AU - Srivastava, Deepak P.

AU - Forster, Robert

AU - Vettenburg, Tom

PY - 2020/1/19

Y1 - 2020/1/19

N2 - We demonstrate the first planar Airy light-sheet microscope. Fluorescence light-sheet microscopy has become the method of choice to study large biological samples with cellular or sub-cellular resolution. The propagation-invariant Airy beam enables a ten-fold increase in field-of-view with single-photon excitation; however, the characteristic asymmetry of the light-sheet limits its potential for multi-photon excitation. Here we show how a planar light-sheet can be formed from the curved propagation-invariant Airy beam. The resulting symmetric light sheet excites two-photon fluorescence uniformly across an extended field-of-view without the need for deconvolution. We demonstrate the method for rapid two-photon imaging of large volumes of neuronal tissue.

AB - We demonstrate the first planar Airy light-sheet microscope. Fluorescence light-sheet microscopy has become the method of choice to study large biological samples with cellular or sub-cellular resolution. The propagation-invariant Airy beam enables a ten-fold increase in field-of-view with single-photon excitation; however, the characteristic asymmetry of the light-sheet limits its potential for multi-photon excitation. Here we show how a planar light-sheet can be formed from the curved propagation-invariant Airy beam. The resulting symmetric light sheet excites two-photon fluorescence uniformly across an extended field-of-view without the need for deconvolution. We demonstrate the method for rapid two-photon imaging of large volumes of neuronal tissue.

KW - physics

KW - optics

UR - https://arxiv.org/abs/2001.06829

M3 - Article

ER -

Hosny NA, Seyforth JA, Spickermann G, Mitchell TJ, Almada P, Chesters R et al. A planar Airy beam light-sheet for two-photon microscopy. arXiv. 2020 Jan 19.