A platelet counting technique to study platelet aggregation in whole blood

application to pathologically low platelet counts

A. R. Saniabadi, G. Lowe, J. Belch, J. C. Barbenel, C. D. Forbes

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    In this study, we have used a platelet counting technique to measure platelet aggregation in whole blood from thrombocytopaenic patients who had platelet counts in the range 18 × 109/I-85 × 109/1. Aliquots of blood were incubated with platelet agonists in a shaking water bath at 37°C. Platelet aggregation was quantified by measuring the fall in single platelets counted in an Ultra-Flo 100 Whole Blood Platelet Counter. ADP (2 M), collagen (1 g/ml) and ristocetin (0.5-2 mg/ml) induced a fall in platelet count of 50%, 60% and 80% respectively. It is concluded that the present method can be used as part of the screening procedure to determine platelet function disorders when platelet counts are too low for aggregation studies with the more widely used photometric technique. A. R. Saniabadi, C. D. Forbes, J. Belch, Department of Medicine, Ninewells Hospital, Dundee, G. Lowe, Department of Medicine, Royal Infirmary, Glasgow, J. C. Barbenel, Bioengineering Unit, University of Strathclyde, Glasgow, UK.

    Original languageEnglish
    Pages (from-to)151-153
    Number of pages3
    JournalPlatelets
    Volume1
    Issue number3
    DOIs
    Publication statusPublished - 1990

    Fingerprint

    Platelet Count
    Platelet Aggregation
    Blood Platelets
    Ristocetin
    Hospital Medicine
    Bioengineering
    Baths
    Adenosine Diphosphate
    Collagen
    Medicine
    Water

    Cite this

    Saniabadi, A. R. ; Lowe, G. ; Belch, J. ; Barbenel, J. C. ; Forbes, C. D. / A platelet counting technique to study platelet aggregation in whole blood : application to pathologically low platelet counts. In: Platelets. 1990 ; Vol. 1, No. 3. pp. 151-153.
    @article{732d68069335455b9e303b0b95933316,
    title = "A platelet counting technique to study platelet aggregation in whole blood: application to pathologically low platelet counts",
    abstract = "In this study, we have used a platelet counting technique to measure platelet aggregation in whole blood from thrombocytopaenic patients who had platelet counts in the range 18 × 109/I-85 × 109/1. Aliquots of blood were incubated with platelet agonists in a shaking water bath at 37°C. Platelet aggregation was quantified by measuring the fall in single platelets counted in an Ultra-Flo 100 Whole Blood Platelet Counter. ADP (2 M), collagen (1 g/ml) and ristocetin (0.5-2 mg/ml) induced a fall in platelet count of 50{\%}, 60{\%} and 80{\%} respectively. It is concluded that the present method can be used as part of the screening procedure to determine platelet function disorders when platelet counts are too low for aggregation studies with the more widely used photometric technique. A. R. Saniabadi, C. D. Forbes, J. Belch, Department of Medicine, Ninewells Hospital, Dundee, G. Lowe, Department of Medicine, Royal Infirmary, Glasgow, J. C. Barbenel, Bioengineering Unit, University of Strathclyde, Glasgow, UK.",
    author = "Saniabadi, {A. R.} and G. Lowe and J. Belch and Barbenel, {J. C.} and Forbes, {C. D.}",
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    doi = "10.3109/09537109009005480",
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    A platelet counting technique to study platelet aggregation in whole blood : application to pathologically low platelet counts. / Saniabadi, A. R.; Lowe, G.; Belch, J.; Barbenel, J. C.; Forbes, C. D.

    In: Platelets, Vol. 1, No. 3, 1990, p. 151-153.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - A platelet counting technique to study platelet aggregation in whole blood

    T2 - application to pathologically low platelet counts

    AU - Saniabadi, A. R.

    AU - Lowe, G.

    AU - Belch, J.

    AU - Barbenel, J. C.

    AU - Forbes, C. D.

    PY - 1990

    Y1 - 1990

    N2 - In this study, we have used a platelet counting technique to measure platelet aggregation in whole blood from thrombocytopaenic patients who had platelet counts in the range 18 × 109/I-85 × 109/1. Aliquots of blood were incubated with platelet agonists in a shaking water bath at 37°C. Platelet aggregation was quantified by measuring the fall in single platelets counted in an Ultra-Flo 100 Whole Blood Platelet Counter. ADP (2 M), collagen (1 g/ml) and ristocetin (0.5-2 mg/ml) induced a fall in platelet count of 50%, 60% and 80% respectively. It is concluded that the present method can be used as part of the screening procedure to determine platelet function disorders when platelet counts are too low for aggregation studies with the more widely used photometric technique. A. R. Saniabadi, C. D. Forbes, J. Belch, Department of Medicine, Ninewells Hospital, Dundee, G. Lowe, Department of Medicine, Royal Infirmary, Glasgow, J. C. Barbenel, Bioengineering Unit, University of Strathclyde, Glasgow, UK.

    AB - In this study, we have used a platelet counting technique to measure platelet aggregation in whole blood from thrombocytopaenic patients who had platelet counts in the range 18 × 109/I-85 × 109/1. Aliquots of blood were incubated with platelet agonists in a shaking water bath at 37°C. Platelet aggregation was quantified by measuring the fall in single platelets counted in an Ultra-Flo 100 Whole Blood Platelet Counter. ADP (2 M), collagen (1 g/ml) and ristocetin (0.5-2 mg/ml) induced a fall in platelet count of 50%, 60% and 80% respectively. It is concluded that the present method can be used as part of the screening procedure to determine platelet function disorders when platelet counts are too low for aggregation studies with the more widely used photometric technique. A. R. Saniabadi, C. D. Forbes, J. Belch, Department of Medicine, Ninewells Hospital, Dundee, G. Lowe, Department of Medicine, Royal Infirmary, Glasgow, J. C. Barbenel, Bioengineering Unit, University of Strathclyde, Glasgow, UK.

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    DO - 10.3109/09537109009005480

    M3 - Article

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    SP - 151

    EP - 153

    JO - Platelets

    JF - Platelets

    SN - 0953-7104

    IS - 3

    ER -