A pore-forming haemolysin from the hookworm, Ancylostoma caninum

Tegan A. Don, Malcolm K. Jones, Danielle Smyth, Peter O'Donoghue, Peter Hotez, Alex Loukas

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

Hookworms feed on blood, but the mechanism by which they lyse ingested erythrocytes is unknown. Here we show that Ancylostoma caninum, the common dog hookworm, expresses a detergent soluble, haemolytic factor. Activity was identified in both adult and larval stages, was heat-stable and unaffected by the addition of protease inhibitors, metal ions, chelators and reducing agents. Trypsin ablated lysis indicating that the haemolysin is a protein. A closely migrating doublet of hookworm proteins with apparent molecular weights of 60-65 kDa bound to the erythrocyte membrane after lysis of cells using both unlabeled and biotinylated detergent-solubilised hookworm extracts. In addition, separation of detergent-soluble parasite extracts using strong cation-exchange chromatography, resulted in purification of 60-65 kDa proteins with trypsin-sensitive haemolytic activity. Erythrocytes lysed with particulate, buffer-insoluble worm extracts were observed using scanning electron microscopy and appeared as red cell ghosts with approximately 100 nm diameter pores formed in the cell membranes. Red blood cell ghosts remained visible indicating that lysis was likely caused by pore formation and followed by osmotic disruption of the cell.

Original languageEnglish
Pages (from-to)1029-1035
Number of pages7
JournalInternational Journal for Parasitology
Volume34
Issue number9
DOIs
Publication statusPublished - 1 Aug 2004

Keywords

  • Ancylostoma caninum
  • Erythrocyte
  • Haemolysin
  • Hookworm
  • Pore-former

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