A potato STRUBBELIG-RECEPTOR FAMILY member, StLRPK1, associates with StSERK3A/BAK1 and activates immunity

Haixia Wang, Yanlin Chen, Xingtong Wu, Zongshang Long, Chunlian Sun, Hairong Wang, Shumei Wang, Paul R. J. Birch, Zhendong Tian (Lead / Corresponding author)

Research output: Contribution to journalArticle

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Abstract

Plant STRUBBELIG (SUB)-RECEPTOR FAMILY (SRF) genes encode putative leucine-rich repeat transmembrane receptor-like kinases. SRFs have been reported to play essential roles in tissue morphogenesis in many plant organs. Here, we show that a potato SRF family gene, StLRPK1, is involved in plant immunity. StLRPK1 is located at the cell plasma membrane and is strongly induced by culture filtrate from in vitro growth of the late blight pathogen Phytophthora infestans. Overexpression of StLRPK1 in stable transgenic potato or ectopic expression in Nicotiana benthamiana plants enhances P. infestans disease resistance, whereas RNA interference (RNAi) of StLRPK1 in potato decreases disease resistance. We found that StLRPK1 constitutively interacts with a pivotal co-receptor, SERK3A/BAK1, which plays a central role in plant immunity. Virus-induced gene silencing of SERK3A/BAK1 in N. benthamiana lines expressing StLRPK1 attenuated P. infestans resistance, indicating that SERK3A/BAK1 is required for StLRPK1-mediated immunity. Finally, we show that StLRPK1-triggered late blight resistance depends on the mitogen-activated protein kinase kinase MEK2 and mitogen-activated protein kinase WIPK. We propose a model in which StLRPK1 associates with SERK3A/BAK1 to positively regulate plant immunity to P. infestans through a MAPK cascade. These data provide new insights into our understanding of SRF function in plant immunity.

Original languageEnglish
Pages (from-to)5573-5586
Number of pages14
JournalJournal of Experimental Botany
Volume69
Issue number22
Early online date22 Aug 2018
DOIs
Publication statusPublished - 1 Dec 2018

Fingerprint

Plant Immunity
Phytophthora infestans
Solanum tuberosum
Immunity
immunity
potatoes
Disease Resistance
receptors
Nicotiana benthamiana
Cell Membrane
MAP Kinase Kinase Kinases
disease resistance
Mitogen-Activated Protein Kinase Kinases
Gene Silencing
RNA Interference
Morphogenesis
Leucine
Genes
Tobacco
mitogen-activated protein kinase kinase

Keywords

  • Late blight
  • MAPK cascade
  • PAMP-triggered immunity
  • receptor-like kinases
  • StLRPK1
  • STRUBBELIG-RECEPTOR FAMILY

Cite this

Wang, Haixia ; Chen, Yanlin ; Wu, Xingtong ; Long, Zongshang ; Sun, Chunlian ; Wang, Hairong ; Wang, Shumei ; Birch, Paul R. J. ; Tian, Zhendong. / A potato STRUBBELIG-RECEPTOR FAMILY member, StLRPK1, associates with StSERK3A/BAK1 and activates immunity. In: Journal of Experimental Botany. 2018 ; Vol. 69, No. 22. pp. 5573-5586.
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abstract = "Plant STRUBBELIG (SUB)-RECEPTOR FAMILY (SRF) genes encode putative leucine-rich repeat transmembrane receptor-like kinases. SRFs have been reported to play essential roles in tissue morphogenesis in many plant organs. Here, we show that a potato SRF family gene, StLRPK1, is involved in plant immunity. StLRPK1 is located at the cell plasma membrane and is strongly induced by culture filtrate from in vitro growth of the late blight pathogen Phytophthora infestans. Overexpression of StLRPK1 in stable transgenic potato or ectopic expression in Nicotiana benthamiana plants enhances P. infestans disease resistance, whereas RNA interference (RNAi) of StLRPK1 in potato decreases disease resistance. We found that StLRPK1 constitutively interacts with a pivotal co-receptor, SERK3A/BAK1, which plays a central role in plant immunity. Virus-induced gene silencing of SERK3A/BAK1 in N. benthamiana lines expressing StLRPK1 attenuated P. infestans resistance, indicating that SERK3A/BAK1 is required for StLRPK1-mediated immunity. Finally, we show that StLRPK1-triggered late blight resistance depends on the mitogen-activated protein kinase kinase MEK2 and mitogen-activated protein kinase WIPK. We propose a model in which StLRPK1 associates with SERK3A/BAK1 to positively regulate plant immunity to P. infestans through a MAPK cascade. These data provide new insights into our understanding of SRF function in plant immunity.",
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author = "Haixia Wang and Yanlin Chen and Xingtong Wu and Zongshang Long and Chunlian Sun and Hairong Wang and Shumei Wang and Birch, {Paul R. J.} and Zhendong Tian",
note = "This study was supported by the grants from the National Natural Science Foundation of China (31171603, 31471550), the National High Technology Research and Development Program of China (2013AA1026/03-05), the Fundamental Research Funds for the Central Universities (2662017PY069) to ZT, China Scholarship Council support to HXW and SW, and the Biotechnology and Biological Sciences Research Council (BBSRC) (BB/N009967/1, BB/L026880/1) to PRJB and SW.",
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A potato STRUBBELIG-RECEPTOR FAMILY member, StLRPK1, associates with StSERK3A/BAK1 and activates immunity. / Wang, Haixia; Chen, Yanlin; Wu, Xingtong; Long, Zongshang; Sun, Chunlian; Wang, Hairong; Wang, Shumei; Birch, Paul R. J.; Tian, Zhendong (Lead / Corresponding author).

In: Journal of Experimental Botany, Vol. 69, No. 22, 01.12.2018, p. 5573-5586.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A potato STRUBBELIG-RECEPTOR FAMILY member, StLRPK1, associates with StSERK3A/BAK1 and activates immunity

AU - Wang, Haixia

AU - Chen, Yanlin

AU - Wu, Xingtong

AU - Long, Zongshang

AU - Sun, Chunlian

AU - Wang, Hairong

AU - Wang, Shumei

AU - Birch, Paul R. J.

AU - Tian, Zhendong

N1 - This study was supported by the grants from the National Natural Science Foundation of China (31171603, 31471550), the National High Technology Research and Development Program of China (2013AA1026/03-05), the Fundamental Research Funds for the Central Universities (2662017PY069) to ZT, China Scholarship Council support to HXW and SW, and the Biotechnology and Biological Sciences Research Council (BBSRC) (BB/N009967/1, BB/L026880/1) to PRJB and SW.

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Plant STRUBBELIG (SUB)-RECEPTOR FAMILY (SRF) genes encode putative leucine-rich repeat transmembrane receptor-like kinases. SRFs have been reported to play essential roles in tissue morphogenesis in many plant organs. Here, we show that a potato SRF family gene, StLRPK1, is involved in plant immunity. StLRPK1 is located at the cell plasma membrane and is strongly induced by culture filtrate from in vitro growth of the late blight pathogen Phytophthora infestans. Overexpression of StLRPK1 in stable transgenic potato or ectopic expression in Nicotiana benthamiana plants enhances P. infestans disease resistance, whereas RNA interference (RNAi) of StLRPK1 in potato decreases disease resistance. We found that StLRPK1 constitutively interacts with a pivotal co-receptor, SERK3A/BAK1, which plays a central role in plant immunity. Virus-induced gene silencing of SERK3A/BAK1 in N. benthamiana lines expressing StLRPK1 attenuated P. infestans resistance, indicating that SERK3A/BAK1 is required for StLRPK1-mediated immunity. Finally, we show that StLRPK1-triggered late blight resistance depends on the mitogen-activated protein kinase kinase MEK2 and mitogen-activated protein kinase WIPK. We propose a model in which StLRPK1 associates with SERK3A/BAK1 to positively regulate plant immunity to P. infestans through a MAPK cascade. These data provide new insights into our understanding of SRF function in plant immunity.

AB - Plant STRUBBELIG (SUB)-RECEPTOR FAMILY (SRF) genes encode putative leucine-rich repeat transmembrane receptor-like kinases. SRFs have been reported to play essential roles in tissue morphogenesis in many plant organs. Here, we show that a potato SRF family gene, StLRPK1, is involved in plant immunity. StLRPK1 is located at the cell plasma membrane and is strongly induced by culture filtrate from in vitro growth of the late blight pathogen Phytophthora infestans. Overexpression of StLRPK1 in stable transgenic potato or ectopic expression in Nicotiana benthamiana plants enhances P. infestans disease resistance, whereas RNA interference (RNAi) of StLRPK1 in potato decreases disease resistance. We found that StLRPK1 constitutively interacts with a pivotal co-receptor, SERK3A/BAK1, which plays a central role in plant immunity. Virus-induced gene silencing of SERK3A/BAK1 in N. benthamiana lines expressing StLRPK1 attenuated P. infestans resistance, indicating that SERK3A/BAK1 is required for StLRPK1-mediated immunity. Finally, we show that StLRPK1-triggered late blight resistance depends on the mitogen-activated protein kinase kinase MEK2 and mitogen-activated protein kinase WIPK. We propose a model in which StLRPK1 associates with SERK3A/BAK1 to positively regulate plant immunity to P. infestans through a MAPK cascade. These data provide new insights into our understanding of SRF function in plant immunity.

KW - Late blight

KW - MAPK cascade

KW - PAMP-triggered immunity

KW - receptor-like kinases

KW - StLRPK1

KW - STRUBBELIG-RECEPTOR FAMILY

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U2 - 10.1093/jxb/ery310

DO - 10.1093/jxb/ery310

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EP - 5586

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

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