A proteomic chronology of gene expression through the cell cycle in human myeloid leukemia cells

Tony Ly, Yasmeen Ahmad, Adam Shlien, Dominique Soroka, Allie Mills, Michael J. Emanuele, Michael R. Stratton, Angus I. Lamond (Lead / Corresponding author)

    Research output: Contribution to journalArticlepeer-review

    75 Citations (Scopus)
    122 Downloads (Pure)

    Abstract

    Technological advances have enabled the analysis of cellular protein and RNA levels with unprecedented depth and sensitivity, allowing for an unbiased re-evaluation of gene regulation during fundamental biological processes. Here, we have chronicled the dynamics of protein and mRNA expression levels across a minimally perturbed cell cycle in human myeloid leukemia cells using centrifugal elutriation combined with mass spectrometry-based proteomics and RNA-Seq, avoiding artificial synchronization procedures. We identify myeloid-specific gene expression and variations in protein abundance, isoform expression and phosphorylation at different cell cycle stages. We dissect the relationship between protein and mRNA levels for both bulk gene expression and for over ~6000 genes individually across the cell cycle, revealing complex, gene-specific patterns. This data set, one of the deepest surveys to date of gene expression in human cells, is presented in an online, searchable database, the Encyclopedia of Proteome Dynamics (http://www.peptracker.com/epd/).

    Original languageEnglish
    Article numbere01630
    JournaleLife
    Volume2014
    Issue number3
    DOIs
    Publication statusPublished - 4 Mar 2014

    Fingerprint Dive into the research topics of 'A proteomic chronology of gene expression through the cell cycle in human myeloid leukemia cells'. Together they form a unique fingerprint.

    Cite this