A quantitative liposome microarray to systematically characterize protein-lipid interactions

Antoine Emmanuel Saliba; Ivana Vonkova; Stefano Ceschia; Greg M Findlay; Kenji Maeda; Christian Tischer; Samy Deghou; Vera van Noort; Peer Bork; Tony Pawson; Jan Ellenberg; Anne-Claude Gavin

doi:10.1038/nmeth.2734

A quantitative liposome microarray to systematically characterize protein-lipid interactions

Antoine Emmanuel Saliba, Ivana Vonkova, Stefano Ceschia, Greg M Findlay, Kenji Maeda, Christian Tischer, Samy Deghou, Vera van Noort, Peer Bork, Tony Pawson, Jan Ellenberg, Anne-Claude Gavin (Lead / Corresponding author)

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Abstract

Lipids have a role in virtually all biological processes, acting as structural elements, scaffolds and signaling molecules, but they are still largely under-represented in known biological networks. Here we describe a liposome microarray-based assay (LiMA), a method that measures protein recruitment to membranes in a quantitative, automated, multiplexed and high-throughput manner.

Original language	English
Pages (from-to)	47-50
Number of pages	4
Journal	Nature Methods
Volume	11
Issue number	1
Early online date	24 Nov 2013
DOIs	https://doi.org/10.1038/nmeth.2734
Publication status	Published - Jan 2014

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth.2734

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abstract = "Lipids have a role in virtually all biological processes, acting as structural elements, scaffolds and signaling molecules, but they are still largely under-represented in known biological networks. Here we describe a liposome microarray-based assay (LiMA), a method that measures protein recruitment to membranes in a quantitative, automated, multiplexed and high-throughput manner.",

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AU - Saliba, Antoine Emmanuel

AU - Vonkova, Ivana

AU - Ceschia, Stefano

AU - Findlay, Greg M

AU - Maeda, Kenji

AU - Tischer, Christian

AU - Deghou, Samy

AU - van Noort, Vera

AU - Bork, Peer

AU - Pawson, Tony

AU - Ellenberg, Jan

AU - Gavin, Anne-Claude

PY - 2014/1

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N2 - Lipids have a role in virtually all biological processes, acting as structural elements, scaffolds and signaling molecules, but they are still largely under-represented in known biological networks. Here we describe a liposome microarray-based assay (LiMA), a method that measures protein recruitment to membranes in a quantitative, automated, multiplexed and high-throughput manner.

AB - Lipids have a role in virtually all biological processes, acting as structural elements, scaffolds and signaling molecules, but they are still largely under-represented in known biological networks. Here we describe a liposome microarray-based assay (LiMA), a method that measures protein recruitment to membranes in a quantitative, automated, multiplexed and high-throughput manner.

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A quantitative liposome microarray to systematically characterize protein-lipid interactions

Abstract

ASJC Scopus subject areas

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