A quantitative liposome microarray to systematically characterize protein-lipid interactions

TY - JOUR

T1 - A quantitative liposome microarray to systematically characterize protein-lipid interactions

AU - Saliba, Antoine Emmanuel

AU - Vonkova, Ivana

AU - Ceschia, Stefano

AU - Findlay, Greg M

AU - Maeda, Kenji

AU - Tischer, Christian

AU - Deghou, Samy

AU - van Noort, Vera

AU - Bork, Peer

AU - Pawson, Tony

AU - Ellenberg, Jan

AU - Gavin, Anne-Claude

PY - 2014/1

Y1 - 2014/1

N2 - Lipids have a role in virtually all biological processes, acting as structural elements, scaffolds and signaling molecules, but they are still largely under-represented in known biological networks. Here we describe a liposome microarray-based assay (LiMA), a method that measures protein recruitment to membranes in a quantitative, automated, multiplexed and high-throughput manner.

AB - Lipids have a role in virtually all biological processes, acting as structural elements, scaffolds and signaling molecules, but they are still largely under-represented in known biological networks. Here we describe a liposome microarray-based assay (LiMA), a method that measures protein recruitment to membranes in a quantitative, automated, multiplexed and high-throughput manner.

UR - https://www.scopus.com/pages/publications/84894623102

U2 - 10.1038/nmeth.2734

DO - 10.1038/nmeth.2734

M3 - Article

C2 - 24270602

AN - SCOPUS:84894623102

SN - 1548-7091

VL - 11

SP - 47

EP - 50

JO - Nature Methods

JF - Nature Methods

IS - 1

ER -