A quantitative proteomic analysis of lung epithelial (A549) cells infected with 2009 pandemic influenza A virus using stable isotope labelling with amino acids in cell culture

Brian K. Dove, Rebecca Surtees, Thomas J. H. Bean, Diane Munday, Helen M. Wise, Paul Digard, Miles W. Carroll, Paul Ajuh, John N. Barr (Lead / Corresponding author), Julian A. Hiscox

    Research output: Contribution to journalArticle

    31 Citations (Scopus)

    Abstract

    Influenza A virus is one of the world's major uncontrolled pathogens, causing seasonal epidemics as well as global pandemics. This was evidenced by the recent emergence and now prevalence of the 2009 swine origin pandemic H1N1 influenza A virus. In this study, quantitative proteomics using stable isotope labelling with amino acids in cell culture was used to investigate the changes in the host cell proteome in cells infected with pandemic H1N1 influenza A virus. The study was conducted in A549 cells that retain properties similar to alveolar cells. Several global pathways were affected, including cell cycle regulation and lipid metabolism, and these could be correlated with recentmicroarray analyses of cells infected with influenza A virus. Taken together, both quantitative proteomics and transcriptomic approaches can be used to identify potential cellular proteins whose functions in the virus life cycle could be targeted for chemotherapeutic intervention

    Original languageEnglish
    Pages (from-to)1431-1436
    Number of pages6
    JournalProteomics
    Volume12
    Issue number9
    DOIs
    Publication statusPublished - May 2012

    Cite this

    Dove, B. K., Surtees, R., Bean, T. J. H., Munday, D., Wise, H. M., Digard, P., Carroll, M. W., Ajuh, P., Barr, J. N., & Hiscox, J. A. (2012). A quantitative proteomic analysis of lung epithelial (A549) cells infected with 2009 pandemic influenza A virus using stable isotope labelling with amino acids in cell culture. Proteomics, 12(9), 1431-1436. https://doi.org/10.1002/pmic.201100470