A Quantitative Study of In Vivo Protoporphyrin IX Fluorescence Build Up During Occlusive Treatment Phases

C. Louise Campbell, C. Tom A. Brown, Kenneth Wood, Ana Gabriela Salvio, Natalia M. Inada, Vanderlei S Bagnato, Harry Moseley (Lead / Corresponding author)

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Abstract

BACKGROUND: Topical photodynamic therapy (PDT) is a non-invasive light based therapy used to treat non-melanoma skin cancer (NMSC) and dysplasia. During PDT, the light sensitive molecule protoporphyrin IX (PpIX) is activated, resulting in the production of singlet oxygen, which subsequently leads to cell death. PpIX is metabolised from a topically applied pro-drug and the strong fluorescence signal associated with PpIX can be utilised as an indicator of the amount of PpIX present within the tumour tissue. In this work we measure the build up PpIX during the occlusive treatment phase and investigate how the PpIX production rate is affected by different lesion and patient characteristics.

METHODS: Fluorescence measurements were used to investigate the build up of PpIX within the tumour tissue during the 3 hour long occlusive treatment prior to irradiation. The study included in vivo measurements of 38 lesions from 38 individual patients. Actinic keratosis (AK) and basal cell carcinoma (BCC) were the lesion types included in this study. The resulting data from the study was analysed using generalised linear mixed effects models.

RESULTS: It was found that the surface fluorescence signal linearly increased with occlusive treatment time. The predictive models suggest that there is a significant difference in PpIX production between lesion location, however no significant difference is demonstrated between different lesion types, gender and skin type.

CONCLUSIONS: The study extends and supports previous knowledge of PpIX production during the occlusive treatment phase.

Original languageEnglish
Pages (from-to)204-207
JournalPhotodiagnosis and photodynamic therapy
Volume18
Early online date28 Feb 2017
DOIs
Publication statusPublished - Jun 2017

Fingerprint

Fluorescence
Therapeutics
Photochemotherapy
Actinic Keratosis
protoporphyrin IX
Light
Singlet Oxygen
Basal Cell Carcinoma
Prodrugs
Skin Neoplasms
Neoplasms
Cell Death
Skin

Cite this

Campbell, C. L., Brown, C. T. A., Wood, K., Salvio, A. G., Inada, N. M., Bagnato, V. S., & Moseley, H. (2017). A Quantitative Study of In Vivo Protoporphyrin IX Fluorescence Build Up During Occlusive Treatment Phases. Photodiagnosis and photodynamic therapy, 18, 204-207. https://doi.org/10.1016/j.pdpdt.2017.02.004
Campbell, C. Louise ; Brown, C. Tom A. ; Wood, Kenneth ; Salvio, Ana Gabriela ; Inada, Natalia M. ; Bagnato, Vanderlei S ; Moseley, Harry. / A Quantitative Study of In Vivo Protoporphyrin IX Fluorescence Build Up During Occlusive Treatment Phases. In: Photodiagnosis and photodynamic therapy. 2017 ; Vol. 18. pp. 204-207.
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abstract = "BACKGROUND: Topical photodynamic therapy (PDT) is a non-invasive light based therapy used to treat non-melanoma skin cancer (NMSC) and dysplasia. During PDT, the light sensitive molecule protoporphyrin IX (PpIX) is activated, resulting in the production of singlet oxygen, which subsequently leads to cell death. PpIX is metabolised from a topically applied pro-drug and the strong fluorescence signal associated with PpIX can be utilised as an indicator of the amount of PpIX present within the tumour tissue. In this work we measure the build up PpIX during the occlusive treatment phase and investigate how the PpIX production rate is affected by different lesion and patient characteristics.METHODS: Fluorescence measurements were used to investigate the build up of PpIX within the tumour tissue during the 3 hour long occlusive treatment prior to irradiation. The study included in vivo measurements of 38 lesions from 38 individual patients. Actinic keratosis (AK) and basal cell carcinoma (BCC) were the lesion types included in this study. The resulting data from the study was analysed using generalised linear mixed effects models.RESULTS: It was found that the surface fluorescence signal linearly increased with occlusive treatment time. The predictive models suggest that there is a significant difference in PpIX production between lesion location, however no significant difference is demonstrated between different lesion types, gender and skin type.CONCLUSIONS: The study extends and supports previous knowledge of PpIX production during the occlusive treatment phase.",
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A Quantitative Study of In Vivo Protoporphyrin IX Fluorescence Build Up During Occlusive Treatment Phases. / Campbell, C. Louise; Brown, C. Tom A.; Wood, Kenneth; Salvio, Ana Gabriela; Inada, Natalia M.; Bagnato, Vanderlei S; Moseley, Harry (Lead / Corresponding author).

In: Photodiagnosis and photodynamic therapy, Vol. 18, 06.2017, p. 204-207.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A Quantitative Study of In Vivo Protoporphyrin IX Fluorescence Build Up During Occlusive Treatment Phases

AU - Campbell, C. Louise

AU - Brown, C. Tom A.

AU - Wood, Kenneth

AU - Salvio, Ana Gabriela

AU - Inada, Natalia M.

AU - Bagnato, Vanderlei S

AU - Moseley, Harry

N1 - C L Campbell acknowledges financial support from an UK EPSRC PhD studentship (EP/K503162/1), the Alfred Stewart Trust, the Russell trust award, the Santander mobility award and the FAPESP CEPOF grant 2013/07276.

PY - 2017/6

Y1 - 2017/6

N2 - BACKGROUND: Topical photodynamic therapy (PDT) is a non-invasive light based therapy used to treat non-melanoma skin cancer (NMSC) and dysplasia. During PDT, the light sensitive molecule protoporphyrin IX (PpIX) is activated, resulting in the production of singlet oxygen, which subsequently leads to cell death. PpIX is metabolised from a topically applied pro-drug and the strong fluorescence signal associated with PpIX can be utilised as an indicator of the amount of PpIX present within the tumour tissue. In this work we measure the build up PpIX during the occlusive treatment phase and investigate how the PpIX production rate is affected by different lesion and patient characteristics.METHODS: Fluorescence measurements were used to investigate the build up of PpIX within the tumour tissue during the 3 hour long occlusive treatment prior to irradiation. The study included in vivo measurements of 38 lesions from 38 individual patients. Actinic keratosis (AK) and basal cell carcinoma (BCC) were the lesion types included in this study. The resulting data from the study was analysed using generalised linear mixed effects models.RESULTS: It was found that the surface fluorescence signal linearly increased with occlusive treatment time. The predictive models suggest that there is a significant difference in PpIX production between lesion location, however no significant difference is demonstrated between different lesion types, gender and skin type.CONCLUSIONS: The study extends and supports previous knowledge of PpIX production during the occlusive treatment phase.

AB - BACKGROUND: Topical photodynamic therapy (PDT) is a non-invasive light based therapy used to treat non-melanoma skin cancer (NMSC) and dysplasia. During PDT, the light sensitive molecule protoporphyrin IX (PpIX) is activated, resulting in the production of singlet oxygen, which subsequently leads to cell death. PpIX is metabolised from a topically applied pro-drug and the strong fluorescence signal associated with PpIX can be utilised as an indicator of the amount of PpIX present within the tumour tissue. In this work we measure the build up PpIX during the occlusive treatment phase and investigate how the PpIX production rate is affected by different lesion and patient characteristics.METHODS: Fluorescence measurements were used to investigate the build up of PpIX within the tumour tissue during the 3 hour long occlusive treatment prior to irradiation. The study included in vivo measurements of 38 lesions from 38 individual patients. Actinic keratosis (AK) and basal cell carcinoma (BCC) were the lesion types included in this study. The resulting data from the study was analysed using generalised linear mixed effects models.RESULTS: It was found that the surface fluorescence signal linearly increased with occlusive treatment time. The predictive models suggest that there is a significant difference in PpIX production between lesion location, however no significant difference is demonstrated between different lesion types, gender and skin type.CONCLUSIONS: The study extends and supports previous knowledge of PpIX production during the occlusive treatment phase.

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DO - 10.1016/j.pdpdt.2017.02.004

M3 - Article

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EP - 207

JO - Photodiagnosis and Photodynamic Therapy

JF - Photodiagnosis and Photodynamic Therapy

SN - 1572-1000

ER -