A role for Snf2-related nucleosome-spacing enzymes in genome-wide nucleosome organization

Triantafyllos Gkikopoulos, Pieta Schofield, Vijender Singh, Marina Pinskaya, Jane Mellor, Michaela Smolle, Jerry L. Workman, Geoffrey J. Barton, Tom Owen-Hughes (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    170 Citations (Scopus)

    Abstract

    The positioning of nucleosomes within the coding regions of eukaryotic genes is aligned with respect to transcriptional start sites. This organization is likely to influence many genetic processes, requiring access to the underlying DNA. Here, we show that the combined action of Isw1 and Chd1 nucleosome-spacing enzymes is required to maintain this organization. In the absence of these enzymes, regular positioning of the majority of nucleosomes is lost. Exceptions include the region upstream of the promoter, the +1 nucleosome, and a subset of locations distributed throughout coding regions where other factors are likely to be involved. These observations indicate that adenosine triphosphate-dependent remodeling enzymes are responsible for directing the positioning of the majority of nucleosomes within the Saccharomyces cerevisiae genome.

    Original languageEnglish
    Pages (from-to)1758-1760
    Number of pages3
    JournalScience
    Volume333
    Issue number6050
    DOIs
    Publication statusPublished - 23 Sep 2011

    Keywords

    • CHROMATIN-REMODELING ENZYME
    • HISTONE-DNA INTERACTIONS
    • IN-VIVO
    • SACCHAROMYCES-CEREVISIAE
    • YEAST ISWI
    • CHD1
    • TRANSCRIPTION
    • IDENTIFICATION
    • MECHANISMS
    • POSITIONS

    Cite this

    Gkikopoulos, T., Schofield, P., Singh, V., Pinskaya, M., Mellor, J., Smolle, M., ... Owen-Hughes, T. (2011). A role for Snf2-related nucleosome-spacing enzymes in genome-wide nucleosome organization. Science, 333(6050), 1758-1760. https://doi.org/10.1126/science.1206097
    Gkikopoulos, Triantafyllos ; Schofield, Pieta ; Singh, Vijender ; Pinskaya, Marina ; Mellor, Jane ; Smolle, Michaela ; Workman, Jerry L. ; Barton, Geoffrey J. ; Owen-Hughes, Tom. / A role for Snf2-related nucleosome-spacing enzymes in genome-wide nucleosome organization. In: Science. 2011 ; Vol. 333, No. 6050. pp. 1758-1760.
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    title = "A role for Snf2-related nucleosome-spacing enzymes in genome-wide nucleosome organization",
    abstract = "The positioning of nucleosomes within the coding regions of eukaryotic genes is aligned with respect to transcriptional start sites. This organization is likely to influence many genetic processes, requiring access to the underlying DNA. Here, we show that the combined action of Isw1 and Chd1 nucleosome-spacing enzymes is required to maintain this organization. In the absence of these enzymes, regular positioning of the majority of nucleosomes is lost. Exceptions include the region upstream of the promoter, the +1 nucleosome, and a subset of locations distributed throughout coding regions where other factors are likely to be involved. These observations indicate that adenosine triphosphate-dependent remodeling enzymes are responsible for directing the positioning of the majority of nucleosomes within the Saccharomyces cerevisiae genome.",
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    author = "Triantafyllos Gkikopoulos and Pieta Schofield and Vijender Singh and Marina Pinskaya and Jane Mellor and Michaela Smolle and Workman, {Jerry L.} and Barton, {Geoffrey J.} and Tom Owen-Hughes",
    year = "2011",
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    doi = "10.1126/science.1206097",
    language = "English",
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    Gkikopoulos, T, Schofield, P, Singh, V, Pinskaya, M, Mellor, J, Smolle, M, Workman, JL, Barton, GJ & Owen-Hughes, T 2011, 'A role for Snf2-related nucleosome-spacing enzymes in genome-wide nucleosome organization', Science, vol. 333, no. 6050, pp. 1758-1760. https://doi.org/10.1126/science.1206097

    A role for Snf2-related nucleosome-spacing enzymes in genome-wide nucleosome organization. / Gkikopoulos, Triantafyllos; Schofield, Pieta; Singh, Vijender; Pinskaya, Marina; Mellor, Jane; Smolle, Michaela; Workman, Jerry L.; Barton, Geoffrey J.; Owen-Hughes, Tom (Lead / Corresponding author).

    In: Science, Vol. 333, No. 6050, 23.09.2011, p. 1758-1760.

    Research output: Contribution to journalArticle

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    AU - Schofield, Pieta

    AU - Singh, Vijender

    AU - Pinskaya, Marina

    AU - Mellor, Jane

    AU - Smolle, Michaela

    AU - Workman, Jerry L.

    AU - Barton, Geoffrey J.

    AU - Owen-Hughes, Tom

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    Gkikopoulos T, Schofield P, Singh V, Pinskaya M, Mellor J, Smolle M et al. A role for Snf2-related nucleosome-spacing enzymes in genome-wide nucleosome organization. Science. 2011 Sep 23;333(6050):1758-1760. https://doi.org/10.1126/science.1206097