A role for the adenomatous Polyposis Coli protein in chromosome segregation

Kenneth B. Kaplan, Aurora A. Burds, Jason R. Swedlow, Songül S. Bekir, Peter K. Sorger, Inke S. Näthke

    Research output: Contribution to journalArticlepeer-review

    449 Citations (Scopus)

    Abstract

    Mutations in the Adenomatous Polyposis Coli (APC) gene are responsible for familial colon cancer and also occur in the early stages of sporadic colon cancer. APC functions in the Wnt signalling pathway to regulate the degradation of beta-catenin (reviewed in refs 1-3). APC also binds to and stabilizes microtubules in vivo and in vitro, localizes to clusters at the ends of microtubules near the plasma membrane of interphase cells, and is an important regulator of cytoskeletal function. Here we show that cells carrying a truncated APC gene (Min) are defective in chromosome segregation. Moreover, during mitosis, APC localizes to the ends of microtubules embedded in kinetochores and forms a complex with the checkpoint proteins Bub1 and Bub3. In vitro, APC is a high-affinity substrate for Bub kinases. Our data are consistent with a role for APC in kinetochore-microtubule attachment and suggest that truncations in APC that eliminate microtubule binding may contribute to chromosomal instability in cancer cells.

    Original languageEnglish
    Pages (from-to)429-432
    Number of pages4
    JournalNature Cell Biology
    Volume3
    Issue number4
    DOIs
    Publication statusPublished - 2001

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