A role for the nuclear envelope in controlling DNA replication within the cell cycle

J. Julian Blow, Ronald A. Laskey

    Research output: Contribution to journalLetter

    441 Citations (Scopus)

    Abstract

    In eukaryotes the entire genome is replicated precisely once in each cell cycle. No DNA is re-replicated until passage through mitosis into the next S-phase. We have used a cell-free DNA replication system from Xenopus eggs to determine which mitotic changes permit DNA to re-replicate. The system efficiently replicates sperm chromatin, but no DNA is re-replicated in a single incubation. This letter shows that nuclei replicated in vitro are unable to re-replicate in fresh replication extract until they have passed through mitosis. However, the only mitotic change which is required to permit re-replication is nuclear envelope permeabilization. This suggests a simple model for the control of DNA replication in the cell cycle, whereby an essential replication factor is unable to cross the nuclear envelope but can only gain access to DNA when the nuclear envelope breaks down at mitosis.
    Original languageEnglish
    Pages (from-to)546-548
    Number of pages3
    JournalNature
    Volume332
    Issue number6164
    DOIs
    Publication statusPublished - 7 Apr 1988

    Fingerprint

    Nuclear Envelope
    DNA Replication
    Cell Cycle
    Mitosis
    DNA
    Xenopus
    Eukaryota
    S Phase
    Eggs
    Chromatin
    Spermatozoa
    Genome

    Cite this

    Blow, J. Julian ; Laskey, Ronald A. / A role for the nuclear envelope in controlling DNA replication within the cell cycle. In: Nature. 1988 ; Vol. 332, No. 6164. pp. 546-548.
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    A role for the nuclear envelope in controlling DNA replication within the cell cycle. / Blow, J. Julian; Laskey, Ronald A.

    In: Nature, Vol. 332, No. 6164, 07.04.1988, p. 546-548.

    Research output: Contribution to journalLetter

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