A separate pool of cardiac phospholemman that does not regulate or associate with the sodium pump: multimers of phospholemman in ventricular muscle

Krzysztof J. Wypijewski, Jacqueline Howie, Louise Reilly, Lindsay B. Tulloch, Karen L. Aughton, Linda M. McLatchie, Michael J. Shattock, Sarah C. Calaghan, William Fuller (Lead / Corresponding author)

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    Abstract

    Phospholemman (PLM), the principal quantitative sarcolemmal substrate for protein kinases A and C in the heart, regulates the cardiac sodium pump. Much like phospholamban, which regulates the related ATPase SERCA, PLM is reported to oligomerise. We investigated subpopulations of PLM in adult rat ventricular myocytes based on phosphorylation status. Co-immunoprecipitation identified two pools of PLM: one not associated with the sodium pump phosphorylated at serine 63 (S63), and one, associated with the pump, both phosphorylated at serine 68 and unphosphorylated. Phosphorylation of PLM at S63 following activation of PKC did not abrogate association of PLM with the pump, so its failure to associate with the pump was not due to phosphorylation at this site. All pools of PLM co-localised to cell surface caveolin-enriched microdomains with sodium pump a subunits, despite the lack of caveolin-binding motif in PLM. Mass spectrometry analysis of phosphospecific immunoprecipitation reactions revealed no unique protein interactions for S63-phosphorylated PLM, and crosslinking reagents also failed to identify any partner proteins for this pool. In lysates from hearts of heterozygous transgenic animals expressing wild type and unphosphorylatable PLM, S63-phosphorylated PLM co-immunoprecipitated unphosphorylatable PLM, confirming the existence of PLM multimers. Dephosphorylation of the PLM multimer does not change Na pump activity. Hence like phospholamban, PLM exists as a pump inhibiting monomer and an un-associated oligomer. The distribution of different PLM phosphorylation states to different pools may be explained by their differential proximity to protein phosphatases, rather than a direct effect of phosphorylation on PLM association with the pump.
    Original languageEnglish
    Pages (from-to)13808-13820
    Number of pages13
    JournalJournal of Biological Chemistry
    Volume288
    Issue number19
    DOIs
    Publication statusPublished - 10 May 2013

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    Wypijewski, K. J., Howie, J., Reilly, L., Tulloch, L. B., Aughton, K. L., McLatchie, L. M., Shattock, M. J., Calaghan, S. C., & Fuller, W. (2013). A separate pool of cardiac phospholemman that does not regulate or associate with the sodium pump: multimers of phospholemman in ventricular muscle. Journal of Biological Chemistry, 288(19), 13808-13820. https://doi.org/10.1074/jbc.M113.460956