A SET/MYND chromatin re-modelling protein regulates Dictyostelium prespore patterning

Beatriz Nunez-Corcuera; Joanna Birch; Jeffrey G. Williams

doi:10.1387/ijdb.113309bn

A SET/MYND chromatin re-modelling protein regulates Dictyostelium prespore patterning

Beatriz Nunez-Corcuera
, Joanna Birch
, Jeffrey G. Williams

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

SmdA is a Dictyosteliumorthologue of the SET/MYND chromatin re-modelling proteins. In developing structures derived from a null mutant for smdA (a smdA- strain), prestalk patterning is normal, but using a prespore lacZ reporter fusion, there is ectopic accumulation of beta-galactosidase in the prestalk region. As wild type slugs migrate, there is continual forward movement and redifferentiation of prespore cells into prestalk cells. Thus, a potential explanation for the ectopic reporter localization in smdA null prestalk cells is an increased rate of re-differentiation and anterior movement of prespore cells. In support of this notion, analysis of an unstable lacZ reporter, driven by the prespore promoter, reveals a normal staining pattern in the smdA- strain. We suggest that one or more genes regulated by SmdA acts to repress prespore re-specification.

Original language	English
Pages (from-to)	205-208
Number of pages	4
Journal	International Journal of Developmental Biology
Volume	55
Issue number	2
DOIs	https://doi.org/10.1387/ijdb.113309bn
Publication status	Published - 2011

Keywords

Dictyostelium
SET/MYND
Prespore
Cell sorting
Histone methyltransferase
Cells
Discoideum
Identification
Transcription
SMYD3
Gene

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10.1387/ijdb.113309bn

Aref#d: 19404. Delineation of the Signalling Pathways that Direct Cellular Differentiation in Dictyostelium (Principal Research Fellowship)
Williams, J. (Investigator)
1/08/08 → 31/07/14
Project: Research

Cite this

@article{1ab7425a8f374f8387afd0b7789cbeec,

title = "A SET/MYND chromatin re-modelling protein regulates Dictyostelium prespore patterning",

abstract = "SmdA is a Dictyosteliumorthologue of the SET/MYND chromatin re-modelling proteins. In developing structures derived from a null mutant for smdA (a smdA- strain), prestalk patterning is normal, but using a prespore lacZ reporter fusion, there is ectopic accumulation of beta-galactosidase in the prestalk region. As wild type slugs migrate, there is continual forward movement and redifferentiation of prespore cells into prestalk cells. Thus, a potential explanation for the ectopic reporter localization in smdA null prestalk cells is an increased rate of re-differentiation and anterior movement of prespore cells. In support of this notion, analysis of an unstable lacZ reporter, driven by the prespore promoter, reveals a normal staining pattern in the smdA- strain. We suggest that one or more genes regulated by SmdA acts to repress prespore re-specification.",

keywords = "Dictyostelium, SET/MYND, Prespore, Cell sorting, Histone methyltransferase, Cells, Discoideum, Identification, Transcription, SMYD3, Gene",

author = "Beatriz Nunez-Corcuera and Joanna Birch and Williams, \{Jeffrey G.\}",

year = "2011",

doi = "10.1387/ijdb.113309bn",

language = "English",

volume = "55",

pages = "205--208",

journal = "International Journal of Developmental Biology",

issn = "0214-6282",

publisher = "UPV/EHU Press",

number = "2",

}

TY - JOUR

T1 - A SET/MYND chromatin re-modelling protein regulates Dictyostelium prespore patterning

AU - Nunez-Corcuera, Beatriz

AU - Birch, Joanna

AU - Williams, Jeffrey G.

PY - 2011

Y1 - 2011

N2 - SmdA is a Dictyosteliumorthologue of the SET/MYND chromatin re-modelling proteins. In developing structures derived from a null mutant for smdA (a smdA- strain), prestalk patterning is normal, but using a prespore lacZ reporter fusion, there is ectopic accumulation of beta-galactosidase in the prestalk region. As wild type slugs migrate, there is continual forward movement and redifferentiation of prespore cells into prestalk cells. Thus, a potential explanation for the ectopic reporter localization in smdA null prestalk cells is an increased rate of re-differentiation and anterior movement of prespore cells. In support of this notion, analysis of an unstable lacZ reporter, driven by the prespore promoter, reveals a normal staining pattern in the smdA- strain. We suggest that one or more genes regulated by SmdA acts to repress prespore re-specification.

AB - SmdA is a Dictyosteliumorthologue of the SET/MYND chromatin re-modelling proteins. In developing structures derived from a null mutant for smdA (a smdA- strain), prestalk patterning is normal, but using a prespore lacZ reporter fusion, there is ectopic accumulation of beta-galactosidase in the prestalk region. As wild type slugs migrate, there is continual forward movement and redifferentiation of prespore cells into prestalk cells. Thus, a potential explanation for the ectopic reporter localization in smdA null prestalk cells is an increased rate of re-differentiation and anterior movement of prespore cells. In support of this notion, analysis of an unstable lacZ reporter, driven by the prespore promoter, reveals a normal staining pattern in the smdA- strain. We suggest that one or more genes regulated by SmdA acts to repress prespore re-specification.

KW - Dictyostelium

KW - SET/MYND

KW - Prespore

KW - Cell sorting

KW - Histone methyltransferase

KW - Cells

KW - Discoideum

KW - Identification

KW - Transcription

KW - SMYD3

KW - Gene

U2 - 10.1387/ijdb.113309bn

DO - 10.1387/ijdb.113309bn

M3 - Article

C2 - 21671223

SN - 0214-6282

VL - 55

SP - 205

EP - 208

JO - International Journal of Developmental Biology

JF - International Journal of Developmental Biology

IS - 2

ER -

A SET/MYND chromatin re-modelling protein regulates Dictyostelium prespore patterning

Abstract

Keywords

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Projects

Aref#d: 19404. Delineation of the Signalling Pathways that Direct Cellular Differentiation in Dictyostelium (Principal Research Fellowship)

Cite this