A simple sequence repeat-based linkage map of Barley

L. Ramsay, M. Macaulay, S. Degli Ivanissevich, K. MacLean, L. Cardle, J. Fuller, K. J. Edwards, S. Tuvesson, M. Morgante, A. Massari, E. Maestri, N. Marmiroli, T. Sjakste, M. Ganal, W. Powell, R. Waugh (Lead / Corresponding author)

Research output: Contribution to journalArticle

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Abstract

A total of 568 new simple sequence repeat (SSR)-based markers for barley have been developed from a combination of database sequences and small insert genomic libraries enriched for a range of short simple sequence repeats. Analysis of the SSRs on 16 barley cultivars revealed variable levels of informativeness but no obvious correlation was found with SSR repeat length, motif type, or map position. Of the 568 SSRs developed, 242 were genetically mapped, 216 with 37 previously published SSRs in a single doubled-haploid population derived from the F1 of an interspecific cross between the cultivar Lina and Hordeum spontaneum Canada Park and 26 SSRs in two other mapping populations. A total of 27 SSRs amplified multiple loci Centromeric clustering of markers was observed in the main mapping population; however, the clustering seventy was reduced in intraspecific crosses, supporting the notion that the observed marker distribution was largely a genetical effect. The mapped SSRs provide a framework for rapidly assigning chromosomal designations and polarity in future mapping programs in barley and a convenient alternative to RFLP for aligning information derived from different populations. A list of the 242 primer pairs that amplify mapped SSRs from total barley genomic DNA is presented.

Original languageEnglish
Pages (from-to)1997-2005
Number of pages9
JournalGenetics
Volume156
Issue number4
Publication statusPublished - 1 Dec 2000

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Hordeum
Microsatellite Repeats
Population
Cluster Analysis
Flax
Genomic Library
Haploidy
Restriction Fragment Length Polymorphisms
Canada
Databases
DNA

Cite this

Ramsay, L., Macaulay, M., Degli Ivanissevich, S., MacLean, K., Cardle, L., Fuller, J., ... Waugh, R. (2000). A simple sequence repeat-based linkage map of Barley. Genetics, 156(4), 1997-2005.
Ramsay, L. ; Macaulay, M. ; Degli Ivanissevich, S. ; MacLean, K. ; Cardle, L. ; Fuller, J. ; Edwards, K. J. ; Tuvesson, S. ; Morgante, M. ; Massari, A. ; Maestri, E. ; Marmiroli, N. ; Sjakste, T. ; Ganal, M. ; Powell, W. ; Waugh, R. / A simple sequence repeat-based linkage map of Barley. In: Genetics. 2000 ; Vol. 156, No. 4. pp. 1997-2005.
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Ramsay, L, Macaulay, M, Degli Ivanissevich, S, MacLean, K, Cardle, L, Fuller, J, Edwards, KJ, Tuvesson, S, Morgante, M, Massari, A, Maestri, E, Marmiroli, N, Sjakste, T, Ganal, M, Powell, W & Waugh, R 2000, 'A simple sequence repeat-based linkage map of Barley', Genetics, vol. 156, no. 4, pp. 1997-2005.

A simple sequence repeat-based linkage map of Barley. / Ramsay, L.; Macaulay, M.; Degli Ivanissevich, S.; MacLean, K.; Cardle, L.; Fuller, J.; Edwards, K. J.; Tuvesson, S.; Morgante, M.; Massari, A.; Maestri, E.; Marmiroli, N.; Sjakste, T.; Ganal, M.; Powell, W.; Waugh, R. (Lead / Corresponding author).

In: Genetics, Vol. 156, No. 4, 01.12.2000, p. 1997-2005.

Research output: Contribution to journalArticle

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AU - Ramsay, L.

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AU - Degli Ivanissevich, S.

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AU - Edwards, K. J.

AU - Tuvesson, S.

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AU - Massari, A.

AU - Maestri, E.

AU - Marmiroli, N.

AU - Sjakste, T.

AU - Ganal, M.

AU - Powell, W.

AU - Waugh, R.

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N2 - A total of 568 new simple sequence repeat (SSR)-based markers for barley have been developed from a combination of database sequences and small insert genomic libraries enriched for a range of short simple sequence repeats. Analysis of the SSRs on 16 barley cultivars revealed variable levels of informativeness but no obvious correlation was found with SSR repeat length, motif type, or map position. Of the 568 SSRs developed, 242 were genetically mapped, 216 with 37 previously published SSRs in a single doubled-haploid population derived from the F1 of an interspecific cross between the cultivar Lina and Hordeum spontaneum Canada Park and 26 SSRs in two other mapping populations. A total of 27 SSRs amplified multiple loci Centromeric clustering of markers was observed in the main mapping population; however, the clustering seventy was reduced in intraspecific crosses, supporting the notion that the observed marker distribution was largely a genetical effect. The mapped SSRs provide a framework for rapidly assigning chromosomal designations and polarity in future mapping programs in barley and a convenient alternative to RFLP for aligning information derived from different populations. A list of the 242 primer pairs that amplify mapped SSRs from total barley genomic DNA is presented.

AB - A total of 568 new simple sequence repeat (SSR)-based markers for barley have been developed from a combination of database sequences and small insert genomic libraries enriched for a range of short simple sequence repeats. Analysis of the SSRs on 16 barley cultivars revealed variable levels of informativeness but no obvious correlation was found with SSR repeat length, motif type, or map position. Of the 568 SSRs developed, 242 were genetically mapped, 216 with 37 previously published SSRs in a single doubled-haploid population derived from the F1 of an interspecific cross between the cultivar Lina and Hordeum spontaneum Canada Park and 26 SSRs in two other mapping populations. A total of 27 SSRs amplified multiple loci Centromeric clustering of markers was observed in the main mapping population; however, the clustering seventy was reduced in intraspecific crosses, supporting the notion that the observed marker distribution was largely a genetical effect. The mapped SSRs provide a framework for rapidly assigning chromosomal designations and polarity in future mapping programs in barley and a convenient alternative to RFLP for aligning information derived from different populations. A list of the 242 primer pairs that amplify mapped SSRs from total barley genomic DNA is presented.

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Ramsay L, Macaulay M, Degli Ivanissevich S, MacLean K, Cardle L, Fuller J et al. A simple sequence repeat-based linkage map of Barley. Genetics. 2000 Dec 1;156(4):1997-2005.