A SUMO-Dependent Protein Network Regulates Chromosome Congression During Oocyte Meiosis

Federico Pelisch, Triin Tammsalu, Bin Wang, Ellis G. Jaffray, Anton Gartner, Ronald T. Hay (Lead / Corresponding author)

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Abstract

During Caenorhabditis elegans oocyte meiosis, a multi-protein ring complex (RC) localised between homologous chromosomes, promotes chromosome congression through the action of the chromokinesin KLP-19. While some RC components are known, the mechanism of RC assembly has remained obscure. We show that SUMO E3 ligase GEI-17/PIAS is required for KLP-19 recruitment to the RC and proteomic analysis identified KLP-19 as a SUMO substrate in vivo. In vitro analysis revealed that KLP-19 is efficiently sumoylated in a GEI-17-dependent manner, while GEI-17 undergoes extensive auto-sumoylation. GEI-17 and another RC component, the kinase BUB-1, contain functional SUMO Interaction Motifs (SIMs) allowing them to recruit SUMO modified proteins, including KLP-19, into the RC. Thus dynamic SUMO modification and the presence of SIMs in RC components generate a SUMO-SIM network that facilitates assembly of the RC. Our results highlight the importance of SUMO-SIM networks in regulating the assembly of dynamic protein complexes.
Original languageEnglish
Pages (from-to)66-77
Number of pages12
JournalMolecular Cell
Volume65
Issue number1
Early online date8 Dec 2016
DOIs
Publication statusPublished - 5 Jan 2017

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Keywords

  • SUMO
  • PIAS
  • meiosis
  • C. elegans
  • SUMO-interaction motif
  • kinesin
  • spindle
  • chromosomes

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