A SUMO-Dependent Protein Network Regulates Chromosome Congression During Oocyte Meiosis

Federico Pelisch, Triin Tammsalu, Bin Wang, Ellis G. Jaffray, Anton Gartner, Ronald T. Hay (Lead / Corresponding author)

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    During Caenorhabditis elegans oocyte meiosis, a multi-protein ring complex (RC) localised between homologous chromosomes, promotes chromosome congression through the action of the chromokinesin KLP-19. While some RC components are known, the mechanism of RC assembly has remained obscure. We show that SUMO E3 ligase GEI-17/PIAS is required for KLP-19 recruitment to the RC and proteomic analysis identified KLP-19 as a SUMO substrate in vivo. In vitro analysis revealed that KLP-19 is efficiently sumoylated in a GEI-17-dependent manner, while GEI-17 undergoes extensive auto-sumoylation. GEI-17 and another RC component, the kinase BUB-1, contain functional SUMO Interaction Motifs (SIMs) allowing them to recruit SUMO modified proteins, including KLP-19, into the RC. Thus dynamic SUMO modification and the presence of SIMs in RC components generate a SUMO-SIM network that facilitates assembly of the RC. Our results highlight the importance of SUMO-SIM networks in regulating the assembly of dynamic protein complexes.
    Original languageEnglish
    Pages (from-to)66-77
    Number of pages12
    JournalMolecular Cell
    Issue number1
    Early online date8 Dec 2016
    Publication statusPublished - 5 Jan 2017


    • SUMO
    • PIAS
    • meiosis
    • C. elegans
    • SUMO-interaction motif
    • kinesin
    • spindle
    • chromosomes


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