A synthetic peptide based on a natural salivary protein reduces demineralisation in model systems for dental caries and erosion

Jelena Kosoric, Ralph Anthony D. Williams, Mark P. Hector, Paul Anderson

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    The salivary protein statherin is an inhibitor of spontaneous and secondary precipitation of hydroxyapatite (HAp). It is also detected in enamel pellicle. The N-terminal region of statherin is involved in its adsorption onto tooth surfaces, and, calcium binding. A peptide (StN21) was designed with a 21 amino acid sequence identical to the N-terminus of statherin. The aim was to measure the effect of StN21 on the rate of mineral loss in a model system for dental caries and erosion using HAp subjected to artificial carious and erosive conditions. StN21 was synthesised using Fmoc chemistry. A surface of each HAp block was exposed to solution containing StN21 at concentrations 9.4-376 μmol L-1 (in phosphate buffer) for 24 h. Controls were HAp exposed to buffer only, and HAp exposed to lysozyme. Demineralising solution (0.1 mol L-1 acetic acid, pH 4.5, 1.0 mmol L-1 calcium and 0.6 mmol L-1 phosphate) was circulated past the HAp blocks at 0.4 mL min -1 to mimic carious and erosive conditions. Scanning microradiography was used to measure the rate of mineral loss for demineralisation periods of 3 weeks. The rate of mineral loss of the samples exposed to StN21 was reduced by ∼40% compared to the controls, but no dependence on the concentration of StN21 was observed at the concentrations used. StN21 has been shown to be a potent and stable peptide that has potential as a preventive/therapeutic agent in the treatment of enamel erosion and dental caries.

    Original languageEnglish
    Pages (from-to)497-503
    Number of pages7
    JournalInternational Journal of Peptide Research and Therapeutics
    Volume13
    Issue number4
    DOIs
    Publication statusPublished - 1 Dec 2007

    Keywords

    • Demineralisation
    • Dentistry
    • Hydroxyapatite
    • Saliva
    • Statherin

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