A transcriptional reference map of defence hormone responses in potato

Lea Wiesel, Jayne L. Davis, Linda Milne, Vanesa Redondo Fernandez, Miriam B. Herold, Jill Middlefell Williams, Jenny Morris, Pete E. Hedley, Brian Harrower, Adrian C. Newton, Paul R. J. Birch, Eleanor M. Gilroy, Ingo Hein (Lead / Corresponding author)

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Abstract

Phytohormones are involved in diverse aspects of plant life including the regulation of plant growth, development and reproduction, as well as governing biotic and abiotic stress responses. We have generated a comprehensive transcriptional reference map of the early potato responses to exogenous application of the defence hormones abscisic acid, brassinolides (applied as epibrassinolide), ethylene (applied as the ethylene precursor aminocyclopropanecarboxylic acid), salicylic acid and jasmonic acid (applied as methyl jasmonate). Of the 39000 predicted genes on the microarray, a total of 2677 and 2473 genes were significantly differentially expressed at 1 h and 6 h after hormone treatment, respectively. Specific marker genes newly identified for the early hormone responses in potato include: a homeodomain 20 transcription factor (DMG400000248) for abscisic acid; a SAUR gene (DMG400016561) induced in epibrassinolide treated plants; an osmotin gene (DMG400003057) specifically enhanced by aminocyclopropanecarboxylic acid; a gene weakly similar to AtWRKY40 (DMG402007388) that was induced by salicylic acid; and a jasmonate ZIM-domain protein 1 (DMG400002930) which was specifically activated by methyl jasmonate. An online database has been set up to query the expression patterns of potato genes represented on the microarray that can also incorporate future microarray or RNAseq-based expression studies.

Original languageEnglish
Article number15229
Number of pages12
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - 19 Oct 2015

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    Wiesel, L., Davis, J. L., Milne, L., Redondo Fernandez, V., Herold, M. B., Middlefell Williams, J., Morris, J., Hedley, P. E., Harrower, B., Newton, A. C., Birch, P. R. J., Gilroy, E. M., & Hein, I. (2015). A transcriptional reference map of defence hormone responses in potato. Scientific Reports, 5, [15229]. https://doi.org/10.1038/srep15229